Plasmonic nanoparticles can be utilized as surface-enhanced Raman scattering (SERS) probes for bioimaging and as photothermal (PT) agents for cancer therapy. Typically, their SERS and PT efficiencies reach maximal values under the on-resonant condition, when the excitation wavelength overlaps the localized surface plasmon resonance (LSPR) wavelength preferably in the near-infrared (NIR) biological window. However, the photogenerated heat may inevitably disturb or even destroy biological samples during the imaging process. Herein, we develop ultrabright SERS probes composed of metallic Au@Ag core-shell rodlike nanomatryoshkas (RNMs) with embedded Raman reporters. By rationally controlling the Ag shell thickness, the LSPR of RNMs can be tuned from UV to NIR range, resulting in highly tunable SERS and PT properties. As bright NIR SERS imaging nanoprobes, RNMs with a thick Ag shell are designed for minimal PT damage to the biological targets under the off-resonance condition, as illustrated through monitoring the changes in mitochondrial membrane potential of cancer cells during SERS imaging procedure. By contrast, RNMs with a thin Ag shell are designed as multifunctional NIR theranostic probes that combine enhanced photothermal therapy capability, as exemplified by efficient PT killing of cancer cells, with reduced yet still efficient imaging properties at the on-resonance excitation.
With the increasing need for multi-purpose analysis in the biomedical field, traditional single diagnosis methods cannot meet the requirements. Therefore new multifunctional technologies and materials for the integration of sample collection, sensing and imaging are in great demand. Core-shell nanoparticles offer a unique platform to combine multifunctions in a single particle. In this work, we have constructed a novel type of core-shell superparamagnetic nanoshell (Fe₃O₄@SiO₂@Au), composed of a Fe₃O₄ cluster core, a thin Au shell and a SiO₂ layer in between. The obtained multifunctional nanoparticles combine the magnetic properties and plasmonic optical properties effectively, which were well investigated by a number of experimental characterization methods and theoretical simulations. We have demonstrated that Fe₃O₄@SiO₂@Au nanoparticles can be utilized for two-photon luminescence (TPL) imaging, near-infrared surface-enhanced Raman scattering (NIR SERS) and cell collection by magnetic separation. The TPL intensity could be further greatly enhanced through the plasmon coupling effect in the self-assembled nanoparticle chains, which were triggered by an external magnetic field. In addition, Fe₃O₄@SiO₂@Au nanoparticles may have great potential applications such as enhanced magnetic resonance imaging (MRI) and photo-thermotherapy. Successful combination of multifunctions including magnetic response, biosensing and bioimaging in single nanoparticles allows further manipulation, real-time tracking, and intracellular molecule analysis of live cells at a single-cell level.
Off-resonant gap-enhanced Raman tags (GERTs) show ultrahigh Raman enhancement and photostabilities and therefore can be used as ideal highly photostable nanoprobes for high-speed and high-resolution Raman bioimaging.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.