C. elegans-based activity guided and size-based isolation of antioxidant peptide fractions from a sea cucumber protein hydrolyzate and their bioinformatic characterization.
The peptide components of defatted
walnut (Juglans regia
L.) meal hydrolysate (DWMH) remain unclear, hindering the
investigation of biological mechanisms and exploitation of bioactive
peptides. The present study aims to identify the peptide composition
of DWMH, followed by to evaluate in vitro antioxidant effects of selected
peptides and investigate mechanisms of antioxidative effect. First,
more than 1 000 peptides were identified by de novo sequencing
in DWMH. Subsequently, a scoring method was established to select
promising bioactive peptides by structure based screening. Eight brand
new peptides were selected due to their highest scores in two different
batches of DWMH. All of them showed potent in vitro antioxidant effects
on H2O2-injured nerve cells. Four of them even
possessed significantly stronger effects than DWMH, making the selected
bioactive peptides useful for further research as new bioactive entities.
Two mechanisms of hydroxyl radical scavenging and ROS reduction were
involved in their antioxidative effects at different degrees. The
results showed peptides possessing similar capacity of hydroxyl radical
scavenging or ROS reduction may have significantly different in vitro
antioxidative effects. Therefore, comprehensive consideration of different
antioxidative mechanisms were suggested in selecting antioxidative
peptides from DWMH.
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