Cara sitasi: Damayanti W, Rochima E, Hasan Zahidah. 2016. Aplikasi kitosan sebagai antibakteri pada filet patin selama penyimpanan suhu rendah. Jurnal Pengolahan Hasil Perikanan Indonesia 19(3): 321-328. AbstrakPenelitian ini bertujuan untuk mendapatkan konsentrasi kitosan yang optimal sebagai bahan antibakteri dengan masa simpan filet patin paling lama pada penyimpanan suhu rendah (5-10 o C). Metode yang digunakan yaitu metode eksperimental dengan rancangan acak lengkap, dengan empat perlakuan dan tiga ulangan. Filet patin direndam dengan perlakuan kitosan 0%, 1%, 2% dan 3% selama tiga menit, dikemas dengan styrofoam dan plastik wrap, kemudian disimpan pada suhu rendah. Pengamatan dilakukan pada hari ke-1, 3, 5, 6, 7, 9, 10, 11 dan 12. Parameter yang diamati meliputi total koloni bakteri, derajat keasaman (pH), susut bobot, dan aktivitas antibakteri kitosan menggunakan metode difusi cakram. Hasil penelitian disimpulkan bahwa kitosan 2% adalah konsentrasi yang optimal untuk penyimpanan filet patin pada suhu rendah hingga hari ke-11, dengan total koloni bakteri 6,7 x10 5 cfu/g, pH 6,67 dan susut bobot sebanyak 6,78%. Kitosan juga memiliki kemampuan aktibakteri yang lebih tinggi terhadap Escherichia coli (bakteri Gram negatif) dibandingkan pada Staphylococcus aureus dan Bacillus subtilis (bakteri Gram positif).Kata kunci: antibakteri, filet patin, kitosan, suhu rendah Abstract This research were to obtain the optimal concentration of chitosan as an antibacterial material wich had the longest of storage period of pangasius fillet at low temperature storage (5-10 o C). The method used is an experimental method with a completely randomized design, four treatments and three replications. Pangasius fillet was soaked with chitosan 0%, 1%, 2% and 3% for 3 minutes, then packed with styrofoam and plastic wrap and stored at low teperature. The observation were made on day 1st, 3rd, 5th, 6th ,7th, 9th,10th, 11th and 12th. The parameters observed total bacterial colonies, the degree of acidity (pH), weight loss, and antibacterial activity of chitosan using disc diffusion methods. The conclusion of research was that the 2% chitosan is optimal concentration for pangasius fillet storage at a low temperature until day 11th, with a total 6.7 X10 5 cfu/g of bacterial colonies, pH 6.67 and total 6,78 % of weight loss.Chitosan also has a higher antibacterial capability against Escherichia coli (Gram negative bacteria) than Staphylococcus aureus and Bacillus subtilis (Gram positive bacteria).
This research was conducted in the Laboratory of Fishery Products Processing, Faculty of Fisheries and<br />Marine Science, University of Padjadjaran, Jatinangor. The purpose of this research is to obtain the optimal<br />concentration of chitosan as an antibacterial material wich had the longest of storage period of pangasius<br />fillet at low temperature storage (5-10oC). The method used is an experimental method with a completely<br />randomized design, four treatments and three replications. Pangasius fillet was soaked with chitosan 0%,<br />1%, 2% and 3% for 3 minutes, then packed with styrofoam and plastic wrap and stored at low teperature.<br />The observation were made on day 1st, 3rd, 5th, 6th ,7th, 9th,10th, 11th and 12th. The parameters observed<br />total bacterial colonies, the degree of acidity (pH), weight loss, and antibacterial activity of chitosan using<br />disc diffusion methods. The final conclusion is that the 2% chitosan is optimal concentration for pangasius<br />fillet storage at a low temperature until day 11th, with a total 6.7 X105 cfu/g of bacterial colonies, pH 6.67 and<br />total 6,78 % of weight loss.Chitosan also has a higher antibacterial capability against Escherichia coli (Gram<br />negative bacteria) than Staphylococcus aureus and Bacillus subtilis (Gram positive bacteria).
HighlightsHA-RF disinfestation treatment had better pre-drying effect of rough rice than that of HA treatmentHighest moisture loss (8.7%) was obtained for samples with highest IMC (39.6% d.b.)Initial moisture content (IMC) correlated negatively with milling quality of rough riceHA-RF can be used as a pre-drying method to partially reduce the moisture contentAbstract.Hot air-assisted radio frequency (HA-RF) is an environmentally friendly disinfestation method, which can completely control the insects and meanwhile maintain quality of rough rice. In this study, the pre-drying effect of HA-RF disinfestation treatment of rough rice with different initial moisture contents (IMCs) (16.7%, 24.8%, 34.7%, and 39.6% d.b.) was investigated along with associated quality change. HA-RF treatments had better pre-drying effect of rough rice with various IMCs than that of hot air (HA) treatment and larger electrode gap obtained lower moisture removal. IMCs of rough rice also influenced the moisture removal for HA-RF treatments, and the corresponding moisture loss were 4.3%, 5.3%, 6.1%, and 8.7% for the treated rough rice with IMC of 16.7%, 24.8%, 34.7%, and 39.6%, respectively. More quality loss was observed for the rough rice with higher IMC, especially for broken rate, fissure, and water uptake ratio. HA-RF treatment had no influences on total phenolic acids, protein, and fat contents, but amylose and vitamin E contents were affected. Non-milled rice treated by HA-RF had an obvious decrease in the lightness (L*) except for the samples with highest IMC (39.6%). RF disinfestation treatment can be used as a pre-drying method to partially reduce the moisture content (MC) of rough rice. Keywords: Disinfestation, Moisture content, Pre-drying, Quality, Rough rice.
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