Exosomes are secreted organelles that have the same topology as the cell and bud outward (outward is defined as away from the cytoplasm) from endosome membranes or endosome-like domains of plasma membrane. Here we describe an exosomal protein-sorting pathway in Jurkat T cells that selects cargo proteins on the basis of both higher-order oligomerization (the oligomerization of oligomers) and plasma membrane association, acts on proteins seemingly without regard to their function, sequence, topology, or mechanism of membrane association, and appears to operate independently of class E vacuolar protein-sorting (VPS) function. We also show that higher-order oligomerization is sufficient to target plasma membrane proteins to HIV virus–like particles, that diverse Gag proteins possess exosomal-sorting information, and that higher-order oligomerization is a primary determinant of HIV Gag budding/exosomal sorting. In addition, we provide evidence that both the HIV late domain and class E VPS function promote HIV budding by unexpectedly complex, seemingly indirect mechanisms. These results support the hypothesis that HIV and other retroviruses are generated by a normal, nonviral pathway of exosome biogenesis.

We report the introduction of π-interaction sites into a series of chemically robust metal–organic frameworks (MOFs), MOF-526, -527, and -528, with progressively increased pore size, 1.9–3.7 nm, and the inclusion and release of large organic molecules in water. The mesopores in these MOFs lead to fast adsorption kinetics, whereas the π-interaction between isolated porphyrin units in the MOF backbone and polycyclic structure of the organic guests provides excellent reversibility. Specifically, seven large organic dyes were quantitatively captured by the porphyrin units of these MOFs in a 2:1 molar ratio, exhibiting unprecedented kinetics for MOFs [e.g., 4.54 × 105 L/mol for rhodamine B] at an extremely low concentration (10 ppm) in water. Rotational-echo double-resonance NMR experiments revealed that the distance between the guest molecules and porphyrin units in MOFs was in the range from 3.24 to 3.37 Å, confirming the specific π-interaction. Repetitive and reversible dynamics was achieved in these MOFs for 10 complete inclusion–release cycles without any decay in performance, which is ideally suited for the removal and recycle of large polycyclic organic molecules from water. The performance of MOF-526 rivals that of state-of-the-art carbon and polymers.

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