The aim of this article is to investigate the potential of using sponges as a bioremediator to remove pathogenic bacteria in integrated aquaculture ecosystems. Using the inter-tidal marine sponge Hymeniacidon perleve as a model system, the ability of removing the most common pathogens Escherichia coli and Vibrio anguillarum II in aquaculture waters was screened in laboratory tests. In sterilized natural seawater (SNSW) supplemented with E. coli at (7.0-8.3) x 10(6) cells/mL, H. perleve can remove an average 96% of E.coli within 10.5 h at a filter rate of ca. (7.53-8.03) x 10(7) cells/h x g of fresh sponge in two independent tests. Despite the removal efficiency and filter rate are similar; the clearance rates (CR) vary significantly among individual sponge specimens and between two batches. For the tests on V. anguillarum II in SNSW, about 1.5 g fresh sponges can keep the pathogen growth under control at a lower initial density 3.6 x 10(4) cells/mL of 200 mL water volume. Further tests were done for 24 h using about 12 g fresh sponge in 2-L actual seawater collected from two aquaculture sites that have ca. eightfold difference in pathogenic bacteria load. The concentrations of E. coli, Vibrio, and total bacteria at 24 h in treatment groups were markedly lower, at about 0.9%, 6.2%-34.5%, and 13.7%-22.5%, respectively, of those in the control. Using a fluoresce stain 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, E. coli, and V. anguillarum II cells were stained and fed to sponges in two independent tests. The confocal microscope observation confirmed that the sponges filtering-retained and digested these bacteria by phagocytosis.
To characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge Hymeniacidon perlevis (Montagu 1818) (Porifera: Demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of individual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. The different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. During its annual life cycle, H. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. Field-grown individual sponge samples at different stages were collected over 7 months (March to September 2005). The dimensions of the silica spicules from these samples were microscopically measured and statistically analyzed. This analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. The trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. A new parameter, the maturation degree (MD) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. Silica spiculogenesis during H. perlevis development was delineated by comparing MD and silicatein expression.
Sessile filter-feeding marine sponges (Porifera) have been reported to possess high efficiency in removing bacteria pollution from natural or aquaculture seawater. However, no investigation has been carried out thus far in a true mariculture farm water system. Therefore this study sought to investigate the ability of the marine sponge Hymeniacidon perlevis to bioremediate the bacteria pollution in the intensive aquaculture water system of turbot Scophthalmus maximus. Sponge specimens were hung in fish culture effluent at different temperature to investigate the optimal temperature condition for bacteria removal by H. perlevis. Turbots S. maximus were co-cultured with sponge H. perlevis in 1.5 m(3) of water system at 15-18 degrees C for 6 weeks to control the growth of bacteria. It was found that H. perlevis was able to remove pathogenic bacteria efficiently at 10-20 degrees C, with a maximal removal of 71.4-78.8% of fecal coliform, 73.9-98.7% of pathogenic vibrio, and 75.0-83.7% of total culturable bacteria from fish-culture effluent at 15 degrees C; H. perlevis continuously showed good bioremediation of bacteria pollution in the S. maximus culture water system, achieving removal of 60.0-90.2% of fecal coliform, 37.6-81.6% of pathogenic vibrio, and 45.1-83.9% of total culturable bacteria. The results demonstrate that H. perlevis is an effective bioremediator of bacteria pollution in the turbot S. maximus culture farm water system.
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