We have successfully
fabricated versatile folate-targeted and oxygen/indocyanine
green-loaded lipid nanoparticles (FA-OINPs) for dual-mode imaging-guided
therapy in ovarian cancer cells and subcutaneous xenograft models.
FA-OINPs were demonstrated to have great potential as superb contrast
agents to enhance ultrasound and photoacoustic (US/PA) imaging We
have successfully fabricated versatile folate-targeted and oxygen/indocyanine
green-loaded lipid nanoparticles (FA-OINPs) for dual-mode imaging-guided
therapy in ovarian cancer cells and subcutaneous xenograft models.
FA-OINPs were demonstrated to have great potential as superb contrast
agents to enhance ultrasound and photoacoustic (US/PA) imaging in
vitro and in vivo. Confocal laser scanning microscopy and flow cytometry
analysis verified that FA-OINPs could specifically target SKOV3 ovarian
cancer cells and be endocytosed with a remarkable efficiency. Compared
with other therapeutic options, FA-OINPs exhibited an excellent therapeutic
outcome after exposure to laser and ultrasound. The MTT assay and
flow cytometry analysis confirmed that cytotoxicity effects and apoptosis/necrosis
rates were significantly increased. The fluorescence microscopy and
fluorescence microplate reader detection validated that the generation
of intracellular reactive oxygen species (ROS) was dramatically improved.
Immunohistochemical analyses of tumor tissues demonstrated the enhanced
tumor apoptosis, the decreased vascular endothelial growth factor
(VEGF) and microvascular density (MVD) expression, and the decreased
expression of CD68 after treatment. The presented results suggest
that photo-sonodynamic/photothermal mediated FA-OINPs could provide
a promising strategy for synergistic therapy in ovarian cancer with
the guidance of US/PA dual-mode imaging.
The goal of our study is to evaluate the efficiency and safety of CT-guided percutaneous lung biopsy for the diagnosis of pulmonary fungal infection in patients with hematologic disease. Medical records were retrospectively reviewed for 16 patients with hematologic diseases, who were initially suspected to have pulmonary fungal infection clinically and underwent further diagnostic methods including blood culture, sputum culture and percutaneous lung biopsy. Of the 16 patients, 10 were diagnosed fungal infection (8 aspergillus, 2 mold fungus), 4 chronic organizing pneumonitis, 1 tuberculosis, and 1 Pneumocystis carinii through histological examination after percutaneous lung biopsy. However, the results of blood culture and sputum culture were negative. CT-guided biopsy showed 100% overall accuracy and 62.5% (10/16) fungal infection rate. The biopsy-induced complications encountered were pneumothorax in 3/16 (18.75%) and hemoptysis in 1/16 (6.25%). No serious complication was found in this series. In conclusion, CT-guided percutaneous lung biopsy is an effective and safe method for the diagnosis of pulmonary fungal infection in patients with hematologic diseases.
Chemotherapy and photo-sonodynamic therapy (PSDT) can be combined through drug delivery nano-platforms to enhance the anti-tumor efficacy, however, which is limited by hypoxia in tumor, thereby causing chemotherapy resistance. Perfluoropentane (PFP) has the ability to carry oxygen and to enhance ultrasound or photoacoustic imaging after vaporization. Herein, we constructed a kind of nanoparticles (PTX/ICG and oxygen loaded PLGA nanoparticles (PIO_NPs)), which had PFP core carrying oxygen and PLGA shell loaded indocyanine green (ICG) and paclitaxel (PTX). PIO_NPs harbored good optical stability and the ability to transit phase. Moreover, it could rapidly release PTX and generate ROS under the mediation by near-infrared laser and low-intensity ultrasound. The PIO_NPs enhanced contrast of the ultrasound and PA imaging. In particular, PIO_NPs may be used to monitor and guide treatment for the accumulation of PIO_NPs at tumor site can be observed by PA imaging. Compared with PTX or other nanoparticles, PIO_NPs combined with laser and ultrasound (L.U) significantly induced apoptosis of SKOV3 cells and inhibited SKOV3 tumor growth. Therefore, PIO_NPs are of great potential in cancer imaging and therapy.
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