The frequencies and diversities of human leukocyte antigen (HLA) alleles and haplotypes are representative of ethnicities. Matching HLA alleles is essential for many clinical applications, including blood transfusion, stem cell transplantation, and tissue/organ transplantation. To date, the information about the frequencies and distributions of HLA alleles and haplotypes among the Kinh Vietnamese population is limited because of the small sample size. In this study, more than 3,750 cord blood units from individuals belonging to the Kinh Vietnamese population were genotyped using PCR sequence-specific oligonucleotide (PCR-SSO) for HLA testing. The results of the study demonstrated that the most frequently occurring HLA-A, -B, -C, and -DRB1 alleles were A*11:01 (25%), A*24:02 (12.3%), A*02:01 (11.2); A*03:03 (8.95%), A*02:03 (7.81%), A*29:01 (7.03%); B*15:02 (15.1%), B*46:01 (10.7%), B*58:01 (7.65%), B*38:02 (7.29%); C*08:01 (17.2), C*07:02 (16.2%), C*01:02 (15.2), C*03:02 (8.3%), C*15:05 (6.13); DRB1*12:02 (31.0%), DRB1*09:01 (10.47%), DRB1*15:02 (7.54%); DRB1*07:01 (6.68%), DRB1*10:01 (6.63%), respectively, with the highest allele diversity level observed in locus B (93 alleles). The most frequent haplotypes of two-locus combinations of HLA-A–B, HLA-A–C, HLA-A–DRB1, HLA-B–C, HLA-B–DRB1, and HLA-C–DRB1 haplotypes were A*11:01–B*15:02 (7.63%), A*11:01–C*08:01 (7.98%), A*11:01–DRB1*12:02 (10.56%), B*15:02–C*08:01 (14.0%), B*15:02–DRB1*12:02 (10.47%), and C*08:01–DRB1*12:02 (11.38%), respectively. In addition, the most frequent haplotypes of three- and four-locus sets of HLA-A–B–C, HLA-A–B–DRB1, HLA-A–C–DRB1, HLA-B–C–DRB1, and HLA-A–B–C–DRB1 were A*11:01–B*15:02–C*08:01 (7.57%), A*11:01–B*15:02–DRB1*12:02 (5.39%), A*11:01–C*08:01–DRB1*12:02 (5.54%), B*15:02–C*08:01–DRB1*12:02 (10.21%), and A*11:01–B*15:02–C*08:01–DRB1*12:02 (5.45%), respectively. This study provides critical information on the frequencies and distributions of HLA alleles and haplotypes in the Kinh Vietnamese population, accounting for more than 85% of Vietnamese citizens. It paves the way to establish an umbilical cord blood bank for cord blood transplantation programs in Vietnam.

This article introduces a new class of Hadamard matrix-based mixed-level foldover designs (MLFODs) and an algorithm which facilitates the construction of MLFODs. Our new MLFODs were constructed by converting some 2-level columns of a Hadamard matrix to 3-level ones. Like the 2-level foldover designs (FODs), each new MLFOD was constructed by a half fraction and its foldover. Our Hadamard-matrix based MLFODs are compared with the conference matrix-based FODs of Jones and Nachtsheim (2014) in terms of the Defficiencies and the maximum of the absolute values of the correlation coefficients among the columns of the model matrix. Like the latter, our designs are also definitive in the sense that the estimates of all main effects are unbiased with respect to any active second-order effects. In addition, they require fewer runs and can be used to study the presence of the second-order effects more efficiently. Examples illustrating the use of our new MLFODs are given.

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This paper discusses an algorithmic approach to constructing trend-free and orthogonally-blocked response surface designs. The constructed designs have the main effects, 2-factor interactions and second-order effects being orthogonal or near-orthogonal to the nuisance factors such as the time-trend or the blocking factors. The paper also provides a catalogue of (near-) trend-free Box–Behnkens designs and orthogonally blocked Box–Behnkens designs arranged in rows and columns.

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