BackgroundGiven the challenges of aging populations, calls have been issued for more sustainable urban re-development and implementation of local solutions to address global environmental and healthy aging issues. However, few studies have considered older adults’ daily mobility to better understand how local built and social environments may contribute to healthy aging. Meanwhile, wearable sensors and interactive map-based applications offer novel means for gathering information on people’s mobility, levels of physical activity, or social network structure. Combining such data with classical questionnaires on well-being, physical activity, perceived environments and qualitative assessment of experience of places opens new opportunities to assess the complex interplay between individuals and environments. In line with current gaps and novel analytical capabilities, this research proposes an international research agenda to collect and analyse detailed data on daily mobility, social networks and health outcomes among older adults using interactive web-based questionnaires and wearable sensors.Methods/DesignOur study resorts to a battery of innovative data collection methods including use of a novel multisensor device for collection of location and physical activity, interactive map-based questionnaires on regular destinations and social networks, and qualitative assessment of experience of places. This rich data will allow advanced quantitative and qualitative analyses in the aim to disentangle the complex people-environment interactions linking urban local contexts to healthy aging, with a focus on active living, social networks and participation, and well-being.DiscussionThis project will generate evidence about what characteristics of urban environments relate to active mobility, social participation, and well-being, three important dimensions of healthy aging. It also sets the basis for an international research agenda on built environment and healthy aging based on a shared and comprehensive data collection protocol.

We propose a ridesharing strategy with integrated transit in which a private on-demand mobility service operator may drop off a passenger directly door-to-door, commit to dropping them at a transit station or picking up from a transit station, or to both pickup and drop off at two different stations with different vehicles. We study the effectiveness of online solution algorithms for this proposed strategy. Queueingtheoretic vehicle dispatch and idle vehicle relocation algorithms are customized for the problem. Several experiments are conducted first with a synthetic instance to design and test the effectiveness of this integrated solution method, the influence of different model parameters, and measure the benefit of such cooperation. Results suggest that rideshare vehicle travel time can drop by 40-60% consistently while passenger journey times can be reduced by 50-60% when demand is high. A case study of Long Island commuters to New York City (NYC) suggests having the proposed operating strategy can substantially cut user journey times and operating costs by up to 54% and 60% each for a range of 10-30 taxis initiated per zone. This result shows that there are settings where such service is highly warranted.

Thermus thermophilus strain HB8 is known to have a heterodimeric aspartyl-tRNA(Asn) amidotransferase (Asp-AdT) capable of forming Asn-tRNA(Asn) [Becker, H.D. and Kern, D. (1998) Proc. Natl. Acad. Sci. USA 95, 12832-12837]. Here we show that, like other bacteria, T. thermophilus possesses the canonical set of amidotransferase (AdT) genes (gatA, gatB and gatC). We cloned and sequenced these genes, and constructed an artificial operon for overexpression in Escherichia coli of the thermophilic holoenzyme. The overproduced T. thermophilus AdT can generate Gln-tRNA(Gln) as well as Asn-tRNA(Asn). Thus, the T. thermophilus tRNA-dependent AdT is a dual-specific Asp/Glu-AdT resembling other bacterial AdTs. In addition, we observed that removal of the 44 carboxy-terminal amino acids of the GatA subunit only inhibits the Asp-AdT activity, leaving the Glu-AdT activity of the mutant AdT unaltered; this shows that Asp-AdT and Glu-AdT activities can be mechanistically separated.

Daily mobility has been shown to contribute to the wellbeing of older adults, as it promotes healthy and independent living. However, very little is known about how the complex relationships between locations, geographic environments and daily mobility relate to wellbeing. In the current paper, we rely on the concept of ‘motility’– defined as potential mobility– and the concept of ‘movement’– defined as actual mobility– to take a step forwards in disentangling the relationship between mobility and wellbeing. We further examine how both motility and movement relate to two complementary definitions of wellbeing: hedonic wellbeing as a measurement of happiness, and eudaimonic wellbeing as the actualisation of an individual’s human potential. To investigate this relationship, we draw up a conceptual framework stressing pathways linking mobility to wellbeing, which we empirically test using structural equation modelling on a stratified sample of 470 older adults. We first quantitatively confirm that motility is defined by access, competences, appropriation and attitudes to modes of transportation. We then observe that motility has direct effects on eudaimonic wellbeing and, to a lesser extent, on hedonic wellbeing. Part of the motility effects on wellbeing are mediated by movement. Separating mobility into motility and movement stresses the independent and complementary role that potential and realised mobility play in shaping older adults’ wellbeing.