Highlights d Systems analysis of the innate immune responses in COVID-19 up to 47 days after onset d Surge of CD169 + and depletion of CD16 + CD14 À monocytes early after symptom onset d High levels of inflammatory cytokines and immature neutrophils in severe COVID-19 d Persistent inflammation in late severe cases, while normalization in mild cases
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Recent technological advances allow profiling of tumor samples to an unparalleled level with
respect to molecular and spatial composition as well as treatment response. We describe a
prospective, observational clinical study performed within the Tumor Profiler (TuPro) Consortium
that aims to show the extent to which such comprehensive information leads to advanced
mechanistic insights of a patient's tumor, enables prognostic and predictive biomarker
discovery, and has the potential to support clinical decision making. For this study of melanoma, ovarian carcinoma, and acute myeloid leukemia tumors, in addition to the emerging standard diagnostic approaches of targeted NGS panel sequencing and digital pathology, we perform
extensive characterization using the following exploratory technologies: single-cell genomics
and transcriptomics, proteotyping, CyTOF, imaging CyTOF, pharmacoscopy, and 4i drug response profiling (4i DRP). In this work, we outline the aims of the TuPro study and present preliminary results on the feasibility of using these technologies in clinical practice showcasing
the power of an integrative multi-modal and functional approach for understanding a tumor's
underlying biology and for clinical decision support.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.