The microfiber-optic interferential sensor based on polyaniline (PANI) sensing layer is efficiently performed in pH detection. The refractive index changes of PANI film can be translated into a significant wavelength shift in the interferometric fringe. We demonstrate the feasibility of PANI attached to the fiber surface in studying the interface polymerization method. The sensing performances of an improved sensitivity in acid solution with − 0.54 n m / p H and alkaline solution with 0.28 nm/pH are systematically investigated. By taking advantage of its miniature size and mechanical flexibility, the microfiber interferential sensor might make a promising platform for pH measurement.
Vaginal flora plays a vital role in human papillomavirus (HPV) infection and progression to cancer. To reveal a role of the vaginal flora in HPV persistence and clearance, 90 patients with HPV infection and 45 healthy individuals were enrolled in this study and their vaginal flora were analyzed. Women with HPV infection were treated with Lactobacillus in the vaginal environment as a supplement to interferon therapy. Our results indicated that patients with high risk HPV (Hr-HPV) 16/18 infection had a significantly higher alpha diversity compared with the healthy control (p < 0.01), while there was no significant difference between the non-Hr-HPV16/18 group and the controls (p > 0.05). Patients with multiple HPV infection had insignificantly higher alpha diversity compared with single HPV infection (p > 0.05). The vaginal flora of patients with HPV infection exhibited different compositions when compared to the healthy controls. The dominant bacteria with the highest prevalence in HPV-positive group were Lactobacillus iners (n = 49, 54.44%), and the top 3 dominant bacteria in the HPV-persistent group were Lactobacillus iners (n = 34, 53.13%), Sneathia amnii (n = 9, 14.06%), and Lactobacillus delbrueckii (n = 3, 4.69%). Patients with HPV clearance had significantly lower alpha diversity, and the flora pattern was also different between groups displaying HPV clearance vs. persistence. The patients with persistent HPV infection had significantly higher levels of Bacteroidaceae, Erysipelotrichaceae, Helicobacteraceae, Neisseriaceae, Streptococcaceae (family level), and Fusobacterium, Bacteroides, Neisseria, and Helicobacter (genus level) than patients who had cleared HPV (p < 0.05).ImportanceOur study revealed differences in vaginal flora patterns are associated with HPV persistence and its clearance. Interferon plus probiotics can greatly improve virus clearance in some patients. Distinguishing bacterial features associated with HPV clearance in patients would be helpful for early intervention and reverse persistent infection.
method, [9] electrochemical method, [10] fluorescence spectrometry, [11] photothermal effect method, [12] anodic dissolution voltammetry, [13] and surface plasmon resonance method. [14] However, colorimetric and photothermal methods require a lot of chemical reagents such as Amino-Functionalized Au@G NPs and Chloroauric acid, [9,12] which can easily cause environmental pollution. Both the anodic dissolution voltammetry and surface plasmon resonance methods are expensive because they require the consumption of a certain number of precious metals such as gold. [13,14] Many other ways of fluorescence spectroscopy, photothermal effect, and anodic dissolution voltammetry are complex, longer consuming time, and inconvenient inspection. [11][12][13] Optic-fiber sensors can be made with large bandwidth, low cost, lightweight, anti-electromagnetic interference, chemical inertness, easy to realize multi-band, and remote real-time monitoring capability, which have been widely used for temperature, [15] humidity, [16] refractive index, [17] gas concentration, [18] breath detection [19] and metal ion detection. [20] So far, many fiber optic sensing techniques for detecting metal ions have been proposed, and the main detection methods are intensity detection, [15][16][17]21] plasma, [22] fluorescence effect, [23] and interference wavelength detection. [24] The main trends are the preparation of sensitizing coatings, the use of vernier effects, and the preparation of micron structures. [20] Many structures of optic fiber are suitable for metal ion detection such as Bragg grating, [24] D-shaped fiber, [25] tilted fiber Bragg grating [26] , and microfiber. [27] Among them, optic-microfibers offering the possibility of strong evanescent fields out of fiber cladding for high-sensitive measurement provide a new option to explore more efficient and economical ion concentration sensing applications.In this paper, we present a microfiber interferometer sensor based on a ternary one-piece sensitive film (PVA-APTES-ICA (PVA, Poly(vinyl alcohol); APTES, (3-Aminopropyl)triethoxysilane; ICA, Imidazole-2-carboxaldehyde)) for the detection of copper ion concentration. The sensing structure can effectively enhance the detection capability of copper ions with a sensitivity of up to 7.715 × 10 6 nm M −1 and the detection limit of 0.0576 ppm in the concentration range of 100 to 1000 nM. The sensor has insensitive to temperature with a sensitivity as low as −0.007 nm °C−1 , which can ignore the temperature cross-influence. The sensor simplifies the manufacturing process of traditional sensors. Stability and anti-interference experiments demonstrated the longterm stability and good specificity of the sensor, which has good application prospects.An optic-fiber sensor for detecting the concentration of trace copper ions is proposed using a ternary cross-linked sensitive film coated on the Mach-Zehnder microfiber interferometer surface. The microfiber has a huge evanescent field offering high sensitivity to the ambient refractive index. The chelation...
Background Hepatocellular carcinoma is one of the most common malignancies with extremely high incidence and mortality rates. Although there have been many studies focus on biomarkers study, few have been reported on PBMC RNA profiles of hepatocellular carcinoma. Methods In this study, we attempted to profile the expression of Peripheral Blood Mononuclear Cells (PBMCs) RNA by using RNA-seq technology and compared the transcriptome between hepatocellular carcinoma patients and the healthy controls. 17 patients and 17 healthy controls involved in this study, PBMCs RNA were sequenced. The sequencing data were analyzed with bioinformatics tools and qRT-PCR was used for selected differential expressed gene validation. Results It is showed that 1578 dysregulated genes found including 1334 upregulated genes and 244 downregulated genes. GO enrichment and KEGG analysis denoted most of the differential expressed genes (DEGs) involved in immune response are closely related to hepatocellular carcinoma. Expression of the 6 selected genes (DEGs, SELENBP1, SLC4A1, SLC26A8, HSPA8P4, CALM1, and RPL7p24) were confirmed by qRT-PCR, and higher sensitivity and specificity obtained by ROC analysis of the 6 genes. CALM1 was found gradually decreasing along with the tumor enlarged. Conclusions It is suggested potential biomarker for diagnosis, classification and therapeutic target of hepatocellular carcinomas. This study provided new visions into development of liver cancer and potential efficient clinical diagnosis in the future.
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