Biological and environmental contrasts between aquatic and terrestrial systems have hindered analyses of community and ecosystem structure across Earth's diverse habitats. Ecological stoichiometry provides an integrative approach for such analyses, as all organisms are composed of the same major elements (C, N, P) whose balance affects production, nutrient cycling, and food-web dynamics. Here we show both similarities and differences in the C:N:P ratios of primary producers (autotrophs) and invertebrate primary consumers (herbivores) across habitats. Terrestrial food webs are built on an extremely nutrient-poor autotroph base with C:P and C:N ratios higher than in lake particulate matter, although the N:P ratios are nearly identical. Terrestrial herbivores (insects) and their freshwater counterparts (zooplankton) are nutrient-rich and indistinguishable in C:N:P stoichiometry. In both lakes and terrestrial systems, herbivores should have low growth efficiencies (10-30%) when consuming autotrophs with typical carbon-to-nutrient ratios. These stoichiometric constraints on herbivore growth appear to be qualitatively similar and widespread in both environments.

A widespread increase in the relative abundances of Asterionella formosa and Fragilaria crotonensis has occurred in oligotrophic alpine lakes across the western United States. Previous investigations have suggested that enhanced atmospheric nitrogen (N) deposition is driving these shifts in diatom community structure; however, little information is available on N requirements of these taxa. We examined the distributions of these two taxa in relation to a variety of physicochemical parameters in a suite of lakes situated in the Beartooth Mountain Range (Montana–Wyoming, USA). We also conducted a series of nutrient enrichment experiments to assess the response of these taxa to changes in N, phosphorus (P), and silica (Si) supply. The distributions of both taxa were positively correlated with C:P, N:P, and Si:P seston ratios, revealing that these taxa are abundant when P availability is very low and the supply of N and Si are moderate to high. In the enrichment experiments, both taxa responded strongly to N additions, whereas P or Si enrichment alone had no effect. While these two taxa are indicative of P enrichment in temperate lakes, our results indicate that in these oligotrophic alpine lakes, N enrichment is driving their recent increase.

We assessed phytoplankton dynamics in three lakes in the Greater Yellowstone Ecosystem to better understand the connections between changing environmental conditions and aquatic communities. This work primarily describes the connections between resource availability and phytoplankton seasonal succession in these lakes. We hypothesized that algal species efficient at utilizing a given resource (including N, P, Si, and light) would be correlated with low relative concentrations of those resources. The lakes generally exhibited moderate resource limitation, which is characteristic of lakes in subalpine and subarctic regions. Although in proximity, the lakes all exhibited different resource relationships: Lewis Lake was most P limited, Jackson Lake was most N limited, and Yellowstone Lake exhibited a moderate degree of N limitation along with periodic Si limitation. Mixing depths and light penetration were also variable among lakes. In 1996, spring diatom biomass was dominated by Stephanodiscus minutulus, Asterionella formosa, Aulacoseira subarctica, and Synedra sp. Relative abundances and dominance varied among the lakes. A. formosa and Synedra sp. abundances were positively correlated with total N : total P (TN : TP) levels in an analysis of data from all three lakes. A. subarctica was negatively correlated with TN : TP and all light : nutrient ratios. Species exhibiting late season maxima included Cyclotella bodanica, Fragilaria crotonensis, and Stephanodiscus niagarae. C. bodanica abundances corresponded to high-light/low-N situations, whereas S. niagarae maxima were found in high-TN : TP/low-light conditions. F. crotonensis abundances were most strongly positively correlated with total Si : TP and TN : TP. Environmental correlations were generally in good agreement with the measured physiological requirements of these species. Additionally, local population maxima of major species of diatoms never coincided.Seasonal succession of phytoplankton has been extensively studied and is generally assumed to be a function of changing environmental variables, including but not limited to temperature, light, nutrients, mixing, and herbivory. A number of investigators have attempted to quantitatively correlate these environmental factors with individual species' abundance and relative dominance with a modest degree of success (Lund 1954;Reynolds 1976;Tilman 1977Tilman , 1982Talling 1987;Sommer 1993). Following these and other examples, we analyzed phytoplankton dynamics over one season in three lakes in the Greater Yellowstone Ecosystem (GYE) to determine whether species-specific patterns could be correlated with measured environmental parameters and whether these patterns were consistent with experimental observations of the physiology of particular species of phytoplankton.Mechanistic resource competition theory (Tilman 1982)Acknowledgments We thank J. Bognar for field assistance and Jackson Lake phytoplankton counts. Also, thanks to the entire staff of the National Park Service Aquatic Resources Office in Yellow...

The objective of this study was to examine the differences in the biochemical and elemental stoichiometry of a freshwater centric diatom, Stephanodiscus minutulus (Grun.), under various nutrient regimes. Stephanodiscus minutulus was grown at μ or 22% of μ under limitation by silicon, nitrogen, or phosphorus. Cell sizes for nutrient-limited cultures were significantly smaller than the non-limited cell sizes, with N-limited cells being significantly smaller than all other treatments. Compared with the nutrient-replete treatment, both carbohydrates and lipids increased in Si- and P-limited cells, whereas carbohydrates increased but proteins decreased in N-limited cells. All of the growth-limited cells showed an increase of carbohydrate and triglyceride, and a decrease of cell size and polar lipids as a percentage of total lipids. The non-limited cells also had a significantly higher chl a concentration and galactolipids as a percentage of total lipids than any of the limited treatments, and the low-Si and low-P cells had significantly higher values than the low-N cells. The particulate C concentrations showed significant differences between treatments, with the Si- and P-limited treatments being significantly higher than the N- and non-limited treatments. Particulate Si did not show a strong relationship with any of the parameters measured, and it was the only parameter with no differences between treatments. The low-Si cells had a significantly higher P content (about two times more) than any other treatment, presumably owing to the luxury consumption of P, and a correspondingly high phospholipid concentration. The elemental data showed that S. minutulus had a high P demand with low optimum N:P (4) and Si:P (10) ratios and a C:N:P ratio of 109:16:2.3. The particulate C showed a positive relationship with POM (r = 0.93), dry weight (r = 0.88), lipid (r = 0.87) and protein (r = 0.84, all P < 0.0001). Particulate N showed a positive relationship with galactolipids (r = 0.95), protein (r = 0.90), dry weight (r = 0.78), lipid (r = 0.75), and cell volume (r = 0.64, all P < 0.0001). It is evident that nutrient limitation in the freshwater diatom S. minutulus has pronounced effects on its biochemical and elemental stoichiometry.

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