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One of the major obstacles hindering cartilage repair is the integration of the reparative cartilage with the recipient cartilage. The purpose of this study was to develop an in vitro model that can be conveniently applied to simulate and improve the integration of tissue engineered cartilage with native articular cartilage. This model, a cartilage integration construct, consists of a cartilage explant and isolated chondrocytes. The explant was anchored to agarose gel on a culture plate as agarose gelation at 4 degrees C to seal the gap between the bottom of the explant and culture plate surface. Isolated chondrocytes were added and confined in the defect created in the center of the explant. After 4 weeks of culture, neocartilage containing proteoglycans and type II collagen was formed. Minimal integration occurred between the neocartilage and the cartilage explant, resembling the failure of cartilage integration manifested in experimental and clinical cartilage repair. In this model, agarose gel anchors the explant onto culture plate by altering temperatures and effectively prevents "leakage" of the isolated chondrocytes from the defect of the explant. This model provides a convenient simulation of the cartilage integration process in vitro and has applications in studies of cartilage integration and cartilage tissue engineering.