The
interaction between biofilms and disinfectant in drinking water
distribution systems (DWDS) as well as the role of this interaction
in the formation of disinfection byproducts has been extensively studied
in recent years. In contrast, lysis of cells and/or release of intracellular
biomolecules from inactivated/damaged cells and their fate and implications
are an overlooked aspect of DWDS. In particular, DNA, once released
into DWDS, may persist in water as extracellular DNA (eDNA). In this
study, we report for the first time that the total DNA extracted from
monochloraminated drinking water contains a high fraction of eDNA.
Drinking water samples were obtained from locations 1 (∼20-year-old
pipeline) and 2 (∼7-year-old pipeline) using glass fiber membranes
with a nominal pore size of 0.4 μm. At location 1, 85–386
ng of eDNA was found per liter of sampled water, which accounted for
52 ± 12% of total DNA, while at location 2, 33–58 ng of
eDNA was found per liter of sampled water, accounting for 42 ±
8% of the total DNA. We further showed that the removal of eDNA reduced
α diversity, increased community evenness, and changed the relative
abundance of detected taxa. Our findings lead to future research questions
about the source, fate, and implications of eDNA in DWDS.
Environmental DNA, i.e., DNA directly extracted from environmental samples, has been applied to understand microbial communities in the environments and to monitor contemporary biodiversity in the conservation context. Environmental DNA often contains both intracellular DNA (iDNA) and extracellular DNA (eDNA). eDNA can persist in the environment and complicate environmental DNA sequencing-based analyses of microbial communities and biodiversity. Although several studies acknowledged the impact of eDNA on DNA-based profiling of environmental communities, eDNA is still being neglected or ignored in most studies dealing with environmental samples. In this article, we summarize key findings on eDNA in environmental samples and discuss the methods used to extract and quantify eDNA as well as the importance of eDNA on the interpretation of experimental results. We then suggest several factors to consider when designing experiments and analyzing data to negate or determine the contribution of eDNA to environmental DNA-based community analyses. This field of research will be driven forward by: (i) carefully designing environmental DNA extraction pipelines by taking into consideration technical details in methods for eDNA extraction/removal and membrane-based filtration and concentration; (ii) quantifying eDNA in extracted environmental DNA using multiple methods including qPCR and fluorescent DNA binding dyes; (iii) carefully interpretating effect of eDNA on DNA-based community analyses at different taxonomic levels; and (iv) when possible, removing eDNA from environmental samples for DNA-based community analyses.
The current study was aimed to explore the differential effects on Gram-positive and Gram-negative freshwater sediment bacterial isolates upon exposure to nano-particles and bulk particles of Al2O3 at low concentrations (0.25, 0.5, and 1 mg/L). The Gram-negative Pseudomonas aeruginosa was more susceptible to both the nano-forms and bulk forms than the Gram-positive Bacillus altitudinis. The generation of reactive oxygen species (ROS) and release of lipopolysaccharide due to membrane damage were dependent on the dose of nano-Al2O3. The Fourier transform infrared spectroscopy (FT-IR) studies confirmed the attachment of nano-Al2O3 on bacterial cells, which may lead to subsequent changes in the cell membrane composition and integrity. Internalization of nano-Al2O3 was estimated to be more for P. aeruginosa than for B. altitudinis cells. As a role of defense mechanism, the biofilm formation and production of extracellular polymeric substances (EPSs; polysaccharide and protein) were increased with respect to the concentration of toxicant. Nano-Al2O3 was estimated to cause more DNA damage than the bulk particles in both Gram-positive and Gram-negative bacterial strains.
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