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The presence of dyes is one of the main contributors to the organic load in textile effluents. In this study a mixture of surfactants, produced from animal/vegetable fats, was used to remove the Direct Yellow 27 dye from a synthetic wastewater through an ionic flocculation process. It was evaluated the effect of contact time, temperature, and surfactant concentration on dye removal efficiency. It was also evaluated the kinetics, equilibrium, and diffusion mechanism of the process. The kinetics of the process was well described by both Pseudo‐second order and Elovich models. The transport of dye molecules to the surfactant flocs is controlled by the external layer. Equilibrium data showed a good fit to the Langmuir model. A removal rate of 93% was achieved in a single stage, after 5 h of contact time.