Micromonas pusilla (Butcher) Manton et Parke, a marine prasinophyte, was used to investigate how cell growth and division affect optical properties of phytoplankton over the light:dark cycle. Measurements were made of cell size and concentration, attenuation and absorption coefficients, flow cytometric forward and side light scattering and chl fluorescence, and chl and carbon content. The refractive index was derived from observations and Mie scattering theory. Diel variations occurred, with cells increasing in size, light scattering, and carbon content during daytime photosynthesis and decreasing during nighttime division. Cells averaged 1.6 μm in diameter and exhibited phased division, with 1.3 divisions per day. Scattering changes resulted primarily from changes in cell size and not refractive index; absorption changes were consistent with a negligible package effect. Measurements over the diel cycle suggest that in M. pusilla carbon‐specific attenuation varies with cell size, and this relationship appears to extend to other phytoplankton species. Because M. pusilla is one of the smallest eukaryotic phytoplankton and belongs to a common marine genus, these results will be useful for interpreting in situ light scattering variation. The relationship between forward light scattering (FLS) and volume over the diel cycle for M. pusilla was similar to that determined for a variety of phytoplankton species over a large size range. We propose a method to estimate cellular carbon content directly from FLS, which will improve our estimates of the contribution of different phytoplankton groups to productivity and total carbon content in the oceans.
Binary-black-hole orbits precess when the black-hole spins are misaligned with the binary's orbital angular momentum. The apparently complicated dynamics can in most cases be described as simple precession of the orbital angular momentum about an approximately fixed total angular momentum. However, the imprint of the precession on the observed gravitational-wave signal is yet more complicated, with a nontrivial time-varying dependence on the black-hole dynamics, the binary's orientation and the detector polarization. As a result, it is difficult to predict under which conditions precession effects are measurable in gravitational-wave observations, and their impact on both signal detection and source characterization. We show that the observed waveform can be simplified by decomposing it as a power series in a new precession parameter b ¼ tanðβ=2Þ, where β is the opening angle between the orbital and total angular momenta. The power series is made up of five harmonics, with frequencies that differ by the binary's precession frequency, and individually do not exhibit amplitude and phase modulations. In many cases, the waveform can be well approximated by the two leading harmonics. In this approximation we are able to obtain a simple picture of precession as caused by the beating of two waveforms of similar frequency. This enables us to identify regions of the parameter space where precession is likely to have an observable effect on the waveform, and to propose a new approach to searching for signals from precessing binaries, based upon the two-harmonic approximation.
Variability in upper ocean optical properties is often driven by changes in the particle pool. We investigated the effects of such changes by characterizing individual particles. For particles in natural assemblages, we used a combination of Mie theory and flow cytometry to determine diameter (D), complex refractive index (n ϩ inЈ), and optical cross-sections at 488 nm. Particles were grouped into categories of eukaryotic pico/nanophytoplankton, Synechococcus, heterotrophic prokaryotes, detritus, and minerals to interpret variability in concurrently measured bulk inherent optical properties (IOPs) in New England continental shelf waters during two seasons. The summed contributions of individual particles to phytoplankton absorption and particle scattering were close to values for these properties measured independently using bulk methods (87% and 107%, respectively). In surface waters during both seasons, eukaryotic phytoplankton were responsible for the majority of both total particle absorption and total particle scattering. Mineral particles contributed the most to backscattering (b b ) in the spring, whereas in the summer both mineral and detrital particles were important. Synechococcus and heterotrophic prokaryotes never contributed more than 14% to IOPs. Our findings emphasize that the measurement of nonliving particles, including detritus and minerals, is necessary for understanding variability in b b in the ocean, an important quantity in the interpretation of satellite ocean color.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.