No abstract

The present work describes a simple hands-on experiment kit for colorimetric quantification of ferric (III) ion (Fe 3+ ) in an aqueous medium using anthocyanin extracted from Ruellia tuberosa L. as a green indicator. The extraction of a high amount of anthocyanin was easily accomplished by using only hot water instead of an organic solvent. The formation of the colored Fe 3+ −anthocyanin complex occurred on a homemade 24-well plate and the generated color was captured by a smartphone. The increase in color intensity was measured in the red, green, blue (RGB) system by the ImageJ software under the optimum conditions. The developed method enabled quantification of Fe 3+ at low concentrations with the detection limit of 0.03 mg L −1 and provided the linear range (0.05−2.0 mg L −1 ) with good linearity (R 2 = 0.9985) with Fe 3+ concentration. The concentrations of Fe 3+ in water samples determined by the developed method were not significantly different from those measured with UV−visible spectrophotometry at a 95% confidence level. In addition, the extracted anthocyanin stored at 4 °C was stable for two months. This hands-on experiment was implemented as a 2 h activity for 30 grade-12 students in which they were asked to determine the concentration of Fe 3+ in a water sample using the smartphone-assisted colorimetric method. The students' understanding of the related concepts of oxidation−reduction and determination of iron was collected by a diagnostic conceptual test. Having participated in the experiment, the students were found to have significantly improved understanding of both concepts.

No abstract

A smartphone application, called CAnal, was developed as a colorimetric analyzer in paper-based devices for sensitive and selective determination of mercury(II) in water samples. Measurement on the double layer of a microfluidic paperbased analytical device (μPAD) fabricated by alkyl ketene dimer (AKD)-inkjet printing technique with special design doped with unmodified silver nanoparticles (AgNPs) onto the detection zones was performed by monitoring the gray intensity in the blue channel of AgNPs, which disintegrated when exposed to mercury(II) on μPAD. Under the optimized conditions, the developed approach showed high sensitivity, low limit of detection (0.003 mg L -1 , 3SD blank/slope of the calibration curve), small sample volume uptake (two times of 2 μL), and short analysis time. The linearity range of this technique ranged from 0.01 to 10 mg L -1 (r 2 = 0.993). Furthermore, practical analysis of various water samples was also demonstrated to have acceptable performance that was in agreement with the data from cold vapor atomic absorption spectrophotometry (CV-AAS), a conventional method. The proposed technique allows for a rapid, simple (instant report of the final mercury(II) concentration in water samples via smartphone display), sensitive, selective, and on-site analysis with high sample throughput (48 samples h -1 , n = 3) of trace mercury(II) in water samples, which is suitable for end users who are unskilled in analyzing mercury(II) in water samples.