Under field conditions, various fungicide molecules were validated for their effectiveness on barley (Hordeum vulgare L.) stripe rust Puccinia striiformis f. sp. consecutively for three years under artificial field epiphytotic conditions. Seven fungicides viz., propiconazole 25%EC (tilt @ 0.1%), tebuconazole 25.9% m/m EC (folicur @ 0.1%), triademefon 25%WP (bayleton @ 0.1%), propiconazole 25%EC (tilt @ 0.05%), tebuconazole 25.9% m/m EC (folicur @ 0.05%), triademefon 25%WP (bayleton@ 0.05%), and mancozeb 75%WP (dithane M45 @ 0.2%) with various concentrations were tested for their effectiveness in controlling barley stripe rust severity. All fungicide applications resulted in lower disease severity and higher grain yields than untreated check plots. All the fungicides @ 0.1% concentrations reduced disease severity ranging from 87.8% to 95.6% except Mancozeb @ 0.2% (34.4%). Significant higher yield was obtained with Propiconazole @ 0.1% (26.7 q/ha) followed by Tebuconazole @ 0.1% (25.2 q/ha) and Triademefon @ 0.1% (24.5 q/ha). The present study revealed propiconazole as the most effective fungicide for the control of stripe rust of barley under epiphytotic conditions.
Maize is an important cereal crop in the world for feed, food, fodder, and raw materials of industries. Turcicum leaf blight (TLB) is a major foliar disease that can cause more than 50% yield losses in maize. Considering this, the molecular diversity, population structure, and genome-wide association study (GWAS) for TLB resistance were studied in 288 diverse inbred lines genotyped using 89 polymorphic simple sequence repeats (SSR) markers. These lines werescreened for TLB disease at two hot-spot locations under artificially inoculated conditions. The average percent disease incidence (PDI) calculated for each genotype ranged from 17 (UMI 1201) to 78% (IML 12-22) with an overall mean of 40%. The numbers of alleles detected at a locus ranged from twoto nine,with a total of 388 alleles. The polymorphic information content (PIC) of each marker ranged between 0.04 and 0.86. Out of 89 markers, 47 markers were highly polymorphic (PIC ≥ 0.60). This indicated that the SSR markers used were very informative and suitable for genetic diversity, population structure, and marker-trait association studies.The overall observed homozygosity for highly polymorphic markers was 0.98, which indicated that lines used were genetically pure. Neighbor-joining clustering, factorial analysis, and population structure studies clustered the 288 lines into 3–5 groups. The patterns of grouping were in agreement with the origin and pedigree records of the genotypesto a greater extent.A total of 94.10% lines were successfully assigned to one or another group at a membership probability of ≥0.60. An analysis of molecular variance (AMOVA) revealed highly significant differences among populations and within individuals. Linkage disequilibrium for r2 and D′ between loci ranged from 0 to 0.77 and 0 to 1, respectively. A marker trait association analysis carried out using a general linear model (GLM) and mixed linear model (MLM), identified 15 SSRs markers significantly associated with TLB resistance.These 15 markers were located on almost all chromosomes (Chr) except 7, 8, and 9. The phenotypic variation explained by these loci ranged from 6% (umc1367) to 26% (nc130, phi085). Maximum 7 associated markers were located together on Chr 2 and 5. The selected regions identified on Chr 2 and 5 corroborated the previous studies carried out in the Indian maize germplasm. Further, 11 candidate genes were identified to be associated with significant markers. The identified sources for TLB resistance and associated markers may be utilized in molecular breeding for the development of suitable genotypes.
The experimental material comprising of 31 genotypes along with four checks of barley were evaluated in Randomized Block Design with three replications over two years during Rabi, 2016-17 and Rabi, 2017-18. Sufficient genetic variability was observed for all the characters in pooled over environments. High PCV and GCV (>20%) values alongwith high heritability coupled with high genetic advance was recorded for number of grains per spike, biological yield per plant and seed yield per plant which indicated the predominance of additive gene action. Association study showed significant and positive correlation of biological yield per plant, number of grains per spike and number of tillers per plant with seed yield per plant. Path coefficient analysis also exhibited high positive direct and indirect contributions of these traits towards seed yield per plant indicating further selection based on these traits for effective improvement in barley.
Among, five IPM modules tested against tomato fruit borer and fruit rot on tomato, the IPM module (M3) consisting of use of pheromone traps (@ 12 traps/ha) just after transplanting the tomato crop , Lycopersicon esculentum Miller for monitoring the population of Helicoverpa armigera . followed by three foliar sprays commencing with a mixture of lamba-cyhalothrin 5EC @ 0.8ml/L(0.04%) and Dithane Z-78 (Zineb) @ 2.5g/L (0.25%) after 10 days of appearance of moths in the traps (after 30 days of transplanting) followed by spray with a mixture of Helicide (Ha NPV) 100 LE @ 0.5ml/L+ Indofil M-45 @ 2.5g/L (0.25%) + Gur (0.05%) + Tween 80 (0.05%) after 15 days of first spray followed by spray with a mixture of lamba-cyhalothrin 5EC @ 0.8ml/L(0.04%) and moximate (cymoxanil + mancozeb) @ 0.25% after 15 days of the second spray was found to be most effective in minimizing the infestation of fruit borer and fruit rot diseases with 50.00% and 63.45% reduction over control, respectively. This module was also found to be most economic resulting in highest marketable fruit yield (255.94q/ha) and maximum net returns (Rs.10.36) per rupee spent. The present findings are of immense utility as there will be reduction in number of sprays resulting in the cost of production of tomato crop.
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