Immunohistochemistry of the S phase related proliferating cell nuclear antigen (PCNA) was studied as an alternative to ex-vivo bromodeoxyuridine (BrdU) immunohistochemistry for assessment of human colonic cell proliferation. From 16 subjects without colonic disease biopsy specimens were collected from five different sites along the colorectum and processed for BrdU
Materials and methods
BIOPSY SPECIMENSBiopsy specimens of endoscopically normal colonic mucosa were taken at five different sites from proximal to distal in the colorectum (ascending, transverse, and descending colon, sigmoid, and rectum) of 16 subjects who had had diagnostic colonoscopy because of abdominal complaints and who were found not to have any organic colonic disorders (seven men, nine women, mean age 49 years, range 17 to 76). All specimens were taken by one endoscopist (LGJBE) and completely processed by the same technician (AP-H). Specimens were immediately placed in RPMI 1640 tissue culture medium containing 10% fetal calf serum, 20 [1M 5-fluoro-uracil, 20 FM bromodeoxyuridine (Serva, Heidelberg, Germany), and 0 9% hydrogenperoxide during one hour at 37°C.The composition of the tissue culture medium was based on a 3 x 3 experiment in which optimal conditions for ex vivo labelling of mouse and human tissue with BrdU were determined. Intestinal specimens were incubated in RPMI 1640 medium containing 10% fetal calf serum and concentrations of 1, 2 or 3x 10-) M BrdU and 0 5, 1 or 2 10-5) M 5-fluorouracil. We found that the addition of hydrogen peroxide seemed to improve the label uptake, therefore in this experiment hydrogen peroxide 30% was added to final concentrations of 0-3, 0-9, and 1-8% respectively. Specimens were immersion fixed in 70% ethanol for at least 24 hours. Best results with regard to immunoreactivity and morphology, were obtained by incubation with RPMI 1640 medium containing 10% fetal calf serum, 2x 10-) M 5-fluoro-uracil, and 0-9% hydrogen peroxide.As maximum incorporation of BrdU can be expected after depletion of the endogenous pool 530 on 12 May 2018 by guest. Protected by copyright.
Health-related quality of life assessment can benefit several groups involved with health care. The aim of our study was to assess construct validity, reliability, discriminant ability and sensitivity to change of the Dutch translation of the Inflammatory Bowel Disease Questionnaire (IBDQ), a disease-specific quality-of-life questionnaire. A group of 120 IBD patients completed the IBDQ and two Visual Analogue Scales concerning general well-being and bowel function twice. Fifty-two patients also completed the Short-Form 36 twice. Disease activity was assessed once. Correlations between the IBDQ scores and the global assessments were reasonably high and the IBDQ was shown to be discriminative. High intraclass correlation coefficients between both measurements of the IBDQ were observed in patients who reported no change in bowel complaints. In contrast, significant differences were found between both moments in patients who reported change. The Dutch IBDQ proved to be valid, discriminative and reliable.
During long-term continuous proton pump inhibitor treatment, serum gastrin and CgA levels are significantly elevated compared to H2RA treatment and nontreated dyspeptic controls. H. pylori infection seems to affect gastric ECL cell secretory function. Increased serum CgA values during long-term profound gastric acid inhibition could reflect either gastric enterochromaffin-like cell hyperfunction or proliferative changes.
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