The first imaging results obtained from a small-size synchrotron are reported. The newly developed Compact Light Source produces inverse Compton X-rays at the intersection point of the counter propagating laser and electron beam. The small size of the intersection point gives a highly coherent cone beam with a few milliradian angular divergence and a few percent energy spread. These specifications make the Compact Light Source ideal for a recently developed grating-based differential phase-contrast imaging method.
PurposeTo assess whether grating-based X-ray dark-field imaging can increase the sensitivity of X-ray projection images in the diagnosis of pulmonary emphysema and allow for a more accurate assessment of emphysema distribution.Materials and MethodsLungs from three mice with pulmonary emphysema and three healthy mice were imaged ex vivo using a laser-driven compact synchrotron X-ray source. Median signal intensities of transmission (T), dark-field (V) and a combined parameter (normalized scatter) were compared between emphysema and control group. To determine the diagnostic value of each parameter in differentiating between healthy and emphysematous lung tissue, a receiver-operating-characteristic (ROC) curve analysis was performed both on a per-pixel and a per-individual basis. Parametric maps of emphysema distribution were generated using transmission, dark-field and normalized scatter signal and correlated with histopathology.ResultsTransmission values relative to water were higher for emphysematous lungs than for control lungs (1.11 vs. 1.06, p<0.001). There was no difference in median dark-field signal intensities between both groups (0.66 vs. 0.66). Median normalized scatter was significantly lower in the emphysematous lungs compared to controls (4.9 vs. 10.8, p<0.001), and was the best parameter for differentiation of healthy vs. emphysematous lung tissue. In a per-pixel analysis, the area under the ROC curve (AUC) for the normalized scatter value was significantly higher than for transmission (0.86 vs. 0.78, p<0.001) and dark-field value (0.86 vs. 0.52, p<0.001) alone. Normalized scatter showed very high sensitivity for a wide range of specificity values (94% sensitivity at 75% specificity). Using the normalized scatter signal to display the regional distribution of emphysema provides color-coded parametric maps, which show the best correlation with histopathology.ConclusionIn a murine model, the complementary information provided by X-ray transmission and dark-field images adds incremental diagnostic value in detecting pulmonary emphysema and visualizing its regional distribution as compared to conventional X-ray projections.
There is a large performance gap between conventional, electron-impact X-ray sources and synchrotron radiation sources. Electron-impact X-ray sources are compact, low to moderate cost, widely available and can have high total flux, but have limited tunability (broad spectrum bremsstrahlung plus fixed characteristic lines) and low brightness. By contrast, synchrotron radiation sources provide extremely high brightness (coherent flux), are tunable and can be monochromatized to a very high degree. However, they are very large and expensive, and typically operated as national user facilities with limited access. An Inverse Compton Scattering (ICS) X-ray source can bridge this gap by providing a narrow-band, high flux and tunable X-ray source that fits into a laboratory at a cost of a few percent of a large synchrotron facility. It works by colliding a high-power laser beam with a relativistic electron beam, in which case the backscattered photons have an energy in the X-ray regime. This paper will describe the working principle of the Lyncean Compact Light Source, a storage-ring based ICS source, its unique beam properties and recent developments that are expected to increase flux and brightness by an order of magnitude compared to earlier versions. Furthermore, it will illustrate how such an X-ray source can be the cornerstone of a local X-ray facility serving applications from diffraction and imaging to scattering and spectroscopy. An overview of demonstrated and potential applications will be provided.
X-ray phase and dark-field contrast have recently been the source of much attention in the field of X-ray imaging, as they both contribute new imaging signals based on physical principles that differ from conventional X-ray imaging. With a so-called Talbot grating interferometer, both phase-contrast and dark-field images are obtained simultaneously with the conventional attenuation-based X-ray image, providing three complementary image modalities that are intrinsically registered. Whereas the physical contrast mechanisms behind attenuation and phase contrast are well understood, a formalism to describe the dark-field signal is still in progress. In this article, we report on correlative experimental results obtained with a grating interferometer and with small-angle X-ray scattering. Furthermore, we use a proposed model to quantitatively describe the results, which could be of great importance for future clinical and biomedical applications of grating-based X-ray imaging.
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