ResumoTrata-se de um estudo transversal, descritivo com abordagem quantitativa, com população configurada como censitária. Os instrumentos utilizados foram o Inventário dos Sintomas de Estresse para Adultos de Lipp (ISSL), a Medida de Independência Funcional (MIF), a Versão Brasileira do Questionário de Qualidade de Vida SF-36 e o Questionário de Avaliação da Sobrecarga do Cuidador Informal (QASCI). Possui o objetivo de avaliar a sobrecarga, a qualidade de vida e a presença de estresse em cuidadores das Estratégias de Saúde da Família (ESFs) de Criciúma, no Estado de Santa Catarina. A maior proporção dos cuidadores foram do gênero feminino (90,9%), com idade média de 51,7 (±11,0) Em relação ao grau de parentesco, 45,5% eram filhos(as), e a MIF Global dos sujeitos ao qual era dedicado o cuidado foi de 70,2 (±40,1). Entre os cuidadores, 81,8% apresentaram presença de estresse e evidenciaram sobrecarga elevada. Foi observada uma correlação positiva moderada (rs=0,571) entre a MIF Global e Dor (SF-36), considerada estatisticamente significativa (p=0,001). Voltar à atenção aos cuidadores, entendendo as barreiras físicas, sociais e emocionais envolvidas no ato de cuidar, torna-se imprescindível no âmbito da saúde coletiva, tanto para proporcionar maior qualidade de vida e menor sobrecarga nos cuidadores quanto a quem é cuidado.

INTRODUÇÃO: A hanseníase é uma das doenças mais antigas da humanidade e persiste como problema de saúde pública. O Brasil ocupa o primeiro lugar na incidência e o segundo na prevalência mundial, e nas Américas representa 93% dos casos, conforme dados da Organização Mundial de Saúde de 2008. O objetivo deste estudo é conhecer o perfil dos pacientes com hanseníase nos municípios da Associação dos Municípios da Região Carbonífera (AMREC), no período de 01/01/2001 a 31/12/2007. MÉTODOS: Trata-se de um estudo transversal e retrospectivo realizado na região da AMREC em Santa Catarina, Brasil. A amostra foi do tipo censitária, em que todos os pacientes notificados foram analisados. RESULTADOS: A população foi de 54 pacientes: 57,4% do sexo feminino e 42,6% do sexo masculino. A faixa etária prevalente foi de 30 a 39 anos (31,5%), a raça branca representou 79,6%. A ocupação foi ignorada em 51 casos, e a baciloscopia em 98%. As formas tuberculóide e virchoviana apresentaram frequência de 27,8% cada e na classificação operacional 50% eram multibacilares. O coeficiente de detecção das cidades variou de baixo a muito alto. CONCLUSÕES: Na análise não há predomínio de multi ou paucibacilares, nem de uma forma clinica específica, contudo sabemos que o diagnóstico está ocorrendo tardiamente pela menor porcentagem de casos indeterminados e com predomínio do sexo feminino. Soma-se a isso o fato de haver escassez dos dados nas fichas de notificação, o que impossibilita-nos de mostrar a realidade da população estudada.

We found a high prevalence of HPV DNA in breast cancer. There is strong evidence to suggest that HPV has an important role in the development of breast cancer.

Introduction:The aim of this study was to establish the incidence rates of congenital syphilis in the South Region of Brazil from 2001 to 2009. Methods: Temporal ecological and descriptive study based on the cases recorded by the System of Information of Notifi able Diseases. Results: The incidence of congenital syphilis has been increasing in the South Region of Brazil since 2004; the highest incidence rates were in women who received prenatal care (113.5 new cases per 100,000 births, p<0.001), who were diagnosed with syphilis at pregnancy (69.8 new cases per 100,000 births, p=0.001), and whose partner did not undergo treatment for syphilis (53.1 new cases per 100,000 births, p=0.001). Conclusions: The population of the present study mostly consisted of adult black women with low educational levels who attended prenatal care, who were diagnosed with syphilis during pregnancy, and whose partners were not treated for syphilis. Based on these results, actions are recommended to reduce the incidence of this disease, which is preventable by early diagnosis and appropriate treatment. The present was merely an ecological study; therefore, further investigations are necessary to elucidate the causes of these fi ndings.

ResumoCom a valorização da estética dentária, aliada à atual filosofia conservadora da Odontologia, as técnicas de clareamento apresentaram uma evolução clínica considerável. A alteração de cor dental vem sendo um dos problemas estéticos mais procurados pelos pacientes no consultório odontológico. O emprego das técnicas clareadoras caseiras e em consultório, quando corretamente executadas, possibilitam resultados estéticos satisfatórios. O objetivo deste estudo foi apresentar as técnicas de clareamento dental (caseiro e consultório), por meio de uma revisão narrativa de literatura. Realizou-se uma discussão dos principais procedimentos adotados, comparando-se os efeitos a curto e longo prazo, vantagens, desvantagens e efeitos colaterais. Nesses tratamentos, os principais agentes clareadores utilizados são o peróxido de carbamida e o peróxido de hidrogênio. Disponíveis em diversas concentrações, são responsáveis por promover resultados satisfatórios no branqueamento dos dentes, cabendo ao cirurgião-dentista efetuar uma análise do perfil do paciente, com vistas à escolha da melhor alternativa de tratamento. Mediante revisão apresentada, ambos os tratamentos foram semelhantes. No entanto, algumas peculiaridades devem ser observadas: para pacientes com sensibilidade dental, o mais indicado é o clareamento caseiro; em pacientes com retração gengival, o mais indicado é o de consultório. Descritores: Clareamento dental • Peróxidos • Peróxido de hidrogênio. AbstractWith the appreciation of aesthetic dentistry, combined with the current conservative philosophy of dentistry, the bleaching techniques had considerable clinical progress. The dental discoloration comes being one of the most popular aesthetic problems by patients in the dental office. The use of at-home and in-office bleaching techniques, when properly executed, enable satisfactory esthetic results. The aim of this study was presenting the tooth bleaching techniques (at-home and in-office), through a literature narrative review. It was held a discussion of the main procedures adopted, by comparing the short-term and long-term effects, advantages, disadvantages and side effects. At these treatments, the main used bleaching agents are carbamide peroxide and hydrogen peroxide. Available in varying concentrations, are responsible for promoting satisfactory results in whitening teeth, leaving the dentist perform an analysis of the patient's profile, with a view to choosing the best alternative treatment. Upon review presented, both treatments were similar. However, some peculiarities must be observed: for patients with tooth sensitivity, the most suitable is the at-home bleaching; in patients with gingival recession, the most suitable is the in-office bleaching.

Tendon injuries represent a clinical challenge in regenerative medicine because their natural repair process is complex and inefficient. The high incidence of tendon injuries is frequently associated with sports practice, aging, tendinopathies, hypertension, diabetes mellitus, and the use of corticosteroids. The growing interest of scientists in using adipose-derived mesenchymal stem cells (ADMSC) in repair processes seems to be mostly due to their paracrine and immunomodulatory effects in stimulating specific cellular events. ADMSC activity can be influenced by GDF-5, which has been successfully used to drive tenogenic differentiation of ADMSC in vitro. Thus, we hypothesized that the application of ADMSC in isolation or in association with GDF-5 could improve Achilles tendon repair through the regulation of important remodeling genes expression. Lewis rats had tendons distributed in four groups: Transected (T), transected and treated with ADMSC (ASC) or GDF-5 (GDF5), or with both (ASC+GDF5). In the characterization of cells before application, ADMSC expressed the positive surface markers, CD90 (90%) and CD105 (95%), and the negative marker, CD45 (7%). ADMSC were also differentiated in chondrocytes, osteoblast, and adipocytes. On the 14th day after the tendon injury, GFP-ADMSC were observed in the transected region of tendons in the ASC and ASC+GDF5 groups, and exhibited and/or stimulated a similar genes expression profile when compared to the in vitro assay. ADMSC up-regulated Lox, Dcn, and Tgfb1 genes expression in comparison to T and ASC+GDF5 groups, which contributed to a lower proteoglycans arrangement, and to a higher collagen fiber organization and tendon biomechanics in the ASC group. The application of ADMSC in association with GDF-5 down-regulated Dcn, Gdf5, Lox, Tgfb1, Mmp2, and Timp2 genes expression, which contributed to a lower hydroxyproline concentration, lower collagen fiber organization, and to an improvement of the rats’ gait 24 h after the injury. In conclusion, although the literature describes the benefic effect of GDF-5 for the tendon healing process, our results show that its application, isolated or associated with ADMSC, cannot improve the repair process of partial transected tendons, indicating the higher effectiveness of the application of ADMSC in injured Achilles tendons. Our results show that the application of ADMSC in injured Achilles tendons was more effective in relation to its association with GDF-5.

Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC). The in vivo imaging showed higher quantification of transplanted PKH26-labeled ASC in tendons of FS + ASC compared to ASC on the 14th day after transection. A small number of Iba1 labeled macrophages carrying PKH26 signal, probably due to phagocytosis of dead ASC, were observed in tendons of transected groups. ASC up-regulated the Tenomodulin gene expression in the transection region when compared to N, T and FS groups and the expression of TIMP-2 and Scleraxis genes in relation to the N group. FS group presented a greater organization of collagen fibers, followed by FS + ASC and ASC in comparison to N. Tendons from ASC group presented higher hydroxyproline concentration in relation to N and the transected tendons of T, FS and FS + ASC had a higher amount of collagen I and tenomodulin in comparison to N group. Although no marked differences were observed in the other biomechanical parameters, T group had higher value of maximum load compared to the groups ASC and FS + ASC. In conclusion, the FS kept constant the number of transplanted ASC in the transected region until the 14th day after injury. Our data suggest this FS to be a good scaffold for treatment during tendon repair because it was the most effective one regarding tendon organization recovering, followed by the FS treatment associated with ASC and finally by the transplanted ASC on the 21st day. Further investigations in long-term time points of the tendon repair are needed to analyze if the higher tissue organization found with the FS scaffold will improve the biomechanics of the tendons.

OBJECTIVETo explore the advantages of using artificial neural networks (ANNs) to recognize patterns in colposcopy to classify images in colposcopy.PURPOSETransversal, descriptive, and analytical study of a quantitative approach with an emphasis on diagnosis. The training test e validation set was composed of images collected from patients who underwent colposcopy. These images were provided by a gynecology clinic located in the city of Criciúma (Brazil). The image database (n = 170) was divided; 48 images were used for the training process, 58 images were used for the tests, and 64 images were used for the validation. A hybrid neural network based on Kohonen self-organizing maps and multilayer perceptron (MLP) networks was used.RESULTSAfter 126 cycles, the validation was performed. The best results reached an accuracy of 72.15%, a sensibility of 69.78%, and a specificity of 68%.CONCLUSIONAlthough the preliminary results still exhibit an average efficiency, the present approach is an innovative and promising technique that should be deeply explored in the context of the present study.