Chemical compounds from cocoa, buni, and cinnamons are expected to inhibit the activity of the enzyme α-amylase, α-glucosidase, and proanthocyanidin as mimetic insulin. Cinnamic acid may inhibit the enzyme activity of HMG-CoA reductase, so that provides benefits for people with diabetes mellitus because it can stimulate pancreatic cells to produce insulin. The objective of this study was to evaluate polyphenols and a-amylase inhibitory activity of a multiherbal formulation. The multiherbal extract prepares with aqueous, acetone, and ethanol. Total phenolic content was found to be 236.28 mg of GAE/100 g (cacao fat extract), 217.94 mg of GAE/100 g (cacao free fat extract), 159.61 mg of GAE/100 g (cinnamons extract), and 181 mg of GAE/100 g (buni extract). α-amylase inhibitory activity found to be 88.74 ppm (cacao extract), 85.32 ppm (cinnamons extract), 83,49 ppm (buni extract), and 13.07 ppm (acarbose). All compounds revealed inhibition potential with IC50 when compared to the standard acarbose.
Chocolate spread is often considered as an unhealthy product with low antioxidant activity and phenolic content since it does not contain cocoa liquor. This research aimed to investigate the potential on the physicochemical composition and antioxidant activity of jackfruit (Artocarpus heterophyllus) flour and red palm olein in the chocolate spread formulations. The effect of the jack fruit and red palm olein incorporation on the quality attributes and high nutritional value of chocolate spread was also examined. Physicochemical properties of the fortified chocolate spread, as well as the evaluation of physical parameters in the chocolate spread, were investigated. Melting points were analyzed by DSC (Differential scanning calorimeter) method, colour lightness was analyzed by chromameter hunter method, profile bioactive compound was analyzed by gas chromatography-mass spectrometry (GC-MS), and whilst for viscosity by Brookfield Viscometer method. The analysis results obtained that the sample code F5 (10% jackfruit flour: 26% sugar) contains of 43.47% fat, 0,88% free fatty acids, polyphenol 127 mg/g, 160.16 mg/g carotenoids, 42.75 μg/mL antioxidant activity IC50, and 6.19 degree of acidity per 100 g chocolate spreads and high panelists preference. However, the fortified chocolate spread had different characteristics from each other either in the chemical, physical or sensory properties. The best formulation code is F5 with 10% jackfruit flour, fortification significantly increased the antioxidant activity, carotenoid, polyphenols, viscosity, colour and melting points. Additionally, chocolate spread with 10% of jackfruit flour by-product presented the highest-ranking test rate of the three sensory attributes of aroma, taste and texture. Therefore, the use of jackfruit by-products in the development of chocolate spread is a viable alternative which can be explored for nutritional, technological and sensory purposes by the food industry.
The purple sweet potato starch was the only carbohydrate component that was converted to its monomeric sugar during the conventional saccharification process, while the fiber remained because α-amylase and amyloglucosidase only act on the α-1,4 and α-1,6 glycosidic bonds in starch. To overcome this, xylanase and mannanase are used to convert hemicellulose to monomeric sugars. The optimal conditions in the process must be known to achieve optimal liquid sugar yield. The purpose of this research was to determine how different enzyme types, times, and their interactions affected the total sugar and dextrose equivalent of liquid sugar produced. This study was carried out by first producing purple sweet potato flour and then generating liquid sugar enzymatically. According to the study’s findings, the treatment combining α-amylase and xylanase was the best combination of enzymes in the liquification, with total sugar reaching 90.83 grams/L. In the saccharification process, the best interaction treatment was α-amylase, xylanase, and AMG with 48 hours of saccharification time (171.37 g/L). After 48 hours of saccharification, the sugar concentration reached its highest point and then fluctuated. The polymerization of monosaccharides under acidic conditions, heat, and the presence of water resulted in a decrease in total dissolved sugar concentration.
Enzyme immobilization is a recovery technique that has been studied in several years, using support as a media to help enzyme dissolutions to the reaction substrate. Immobilization method used in this study was adsorption method, using specific lipase fromAspergillus oryzae. Lipase was partially purified from the culture supernatant ofAspergillus oryzae. Enzyme was immobilized by adsorbed on silica gel. Studies on free and immobilized lipase systems for determination of optimum pH, optimum temperature, thermal stability and reusability were carried out. The results showed that free lipase had optimum pH 8,2 and optimum temperature 35 °C while the immobilized lipase had optimum 8,2 and optimum temperature 45 °C. The thermal stability of the immobilized lipase, relative to that of the free lipase, was markedly increased. The immobilized lipase can be reused for at least six times.
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