SUMMARY Flap endonuclease (FEN1), essential for DNA replication and repair, removes RNA and DNA 5′-flaps. FEN1 5′ nuclease superfamily members acting in nucleotide excision repair (XPG), mismatch repair (EXO1) and homologous recombination (GEN1) paradoxically incise structurally distinct bubbles, ends or Holliday junctions, respectively. Here structural and functional analyses of human FEN1:DNA complexes show structure-specific, sequence-independent recognition for nicked dsDNA bent 100° with unpaired 3′- and 5′-flaps. Above the active site, a helical cap over a gateway formed by two helices enforces ssDNA threading and specificity for free 5′-ends. Crystallographic analyses of product and substrate complexes reveal that dsDNA binding and bending, the ssDNA gateway, and double-base unpairing flanking the scissile phosphate control precise flap incision by the two-metal-ion active site. Superfamily conserved motifs bind and open dsDNA, direct the target region into the helical gateway permitting only non-base-paired oligonucleotides active site access, and support a unified understanding of superfamily substrate specificity.

Search for high-mass dilepton resonances using139 fb −1 of p p collision data collected at √ s = 13 TeV with the ATLAS detectorThe ATLAS Collaboration A search for high-mass dielectron and dimuon resonances in the mass range of 250 GeV to 6 TeV is presented. The data were recorded by the ATLAS experiment in proton-proton collisions at a centre-of-mass energy of √ s = 13 TeV during Run 2 of the Large Hadron Collider and correspond to an integrated luminosity of 139 fb −1 . A functional form is fitted to the dilepton invariant-mass distribution to model the contribution from background processes, and a generic signal shape is used to determine the significance of observed deviations from this background estimate. No significant deviation is observed and upper limits are placed at the 95% confidence level on the fiducial cross-section times branching ratio for various resonance width hypotheses. The derived limits are shown to be applicable to spin-0, spin-1 and spin-2 signal hypotheses. For a set of benchmark models, the limits are converted into lower limits on the resonance mass and reach 4.5 TeV for the E 6 -motivated Z ψ boson. Also presented are limits on Heavy Vector Triplet model couplings.ATLAS [14-16] is a multipurpose detector with a forward-backward symmetric cylindrical geometry with respect to the LHC beam axis.1 The innermost layers consist of tracking detectors in the pseudorapidity range |η| < 2.5. This inner detector (ID) is surrounded by a thin superconducting solenoid that provides a 1 ATLAS uses a right-handed coordinate system with its origin at the nominal interaction point (IP) in the centre of the detector and the z-axis along the beam pipe. The x-axis points from the IP to the centre of the LHC ring, and the y-axis points upwards. Cylindrical coordinates (r, φ) are used in the transverse plane, φ being the azimuthal angle around the z-axis. The pseudorapidity is defined in terms of the polar angle θ as η = − ln tan(θ/2). Angular distance is measured in units of ∆R ≡ (∆η) 2 + (∆φ) 2 .

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High-precision measurements by the ATLAS Collaboration are presented of inclusive , and () Drell–Yan production cross sections at the LHC. The data were collected in proton–proton collisions at with an integrated luminosity of . Differential and cross sections are measured in a lepton pseudorapidity range . Differential cross sections are measured as a function of the absolute dilepton rapidity, for , for three intervals of dilepton mass, , extending from 46 to . The integrated and differential electron- and muon-channel cross sections are combined and compared to theoretical predictions using recent sets of parton distribution functions. The data, together with the final inclusive scattering cross-section data from H1 and ZEUS, are interpreted in a next-to-next-to-leading-order QCD analysis, and a new set of parton distribution functions, ATLAS-epWZ16, is obtained. The ratio of strange-to-light sea-quark densities in the proton is determined more accurately than in previous determinations based on collider data only, and is established to be close to unity in the sensitivity range of the data. A new measurement of the CKM matrix element is also provided.