Both techniques, but particularly EASI-MS, provide simple, fast and efficient MS fingerprinting methodologies to characterise the P. pubescens oil with typical (di)terpene profiles being applicable to quality control and certification of authenticity and origin.
BackgroundArtemisia annua L. has been used for many centuries in Chinese traditional medicine. Artemisinin, the active principle was first isolated and identified in the 1970s becoming the global back bone to the fight against malaria. Our research group previously developed an economic and ecological friendly process to obtain this compound. In the pursuit to also exploit the residue generated throughout the process we further evaluated the pharmacological potential of that extract.MethodsThe alcoholic crude extract after artemisinin precipitation maintained an enriched sesquiterpene lactones content with residue artemisinin (1.72%) and deoxyartemisinin (0.31%), used as chemical markers for this sample. This study evaluated the pharmacological potential of the enriched sesquiterpene lactone fraction (Lac-FR) on different nociceptive and inflammatory experimental animal models. Previous findings on the biological properties of lactones obtained from natural products permitted us to explore the antinociceptive activities of these compounds based on in vivo chemical-induced behavioral assays.ResultsThe enriched sesquiterpene lactone fraction (Lac-FR) was administrated by intraperitoneal injection producing a relevant reduction in the reaction time of the animals in both phases of the formalin test, significantly reduced the sensitivity to mechanical allodynia stimulus, reduced the paw edema caused by carrageenan injection and promoted high antinociceptive activity in tail flick model suggesting relationship with the opioid system. Further studies are being undertaken to elucidate the active components involved with the antinociceptive activity as well as evaluation of synergy effect that is seen by combination of substances that is greater than would be expected from consideration of individual contributions.ConclusionFor the first time, results presented herein provided consistent data to support the potential use of these lactones for pain relief as revealed by chemical-induced nociception assays in mice.
One great challenge of head and neck cancer treatment with Cisplatin and Radiotherapy is the development of adverse side effects that diminishes treatment adhesion, among them is Oral Mucositis (OM). The "golden pattern" nowadays for OM treatment in Brasil is the application of low intensity laser, however this treatment is obsolete, since there are not enough trained professionals in this technique. Previous studies with Arrabiadaea chica have proven that the use in healing processes is not only effective, but safe, since no toxic or adverse effetcs were observed in phase I clinical trials with gel formulations containing 2,5% standardized A.chica extract. Therefore, in this second phase clinical trial the comparison of application of 2,5% A.chica gel with low intensity laser treatment were evaluated in patients under treatment of head and neck squamous cells carcinoma that developed OM. Once approves, A. chica formulation will present a simpler, more effective and low cost option for OM treatment of SUS pacients.
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