No abstract

Melatonin profiles in the cerebrospinal fluid (CSF) of conscious goats were examined under long-day (16L:8D) and short-day (8L:16D) environments. CSF melatonin, collected from the lateral ventricle, showed distinct 24 h rhythms with high concentrations being restricted to the dark phase, which averaged 1,320.6 pg/ml under 16L:8D and 660.6 pg/ml under 8L:16D. On the contrary, the nocturnal rise in CSF melatonin was totally absent in the pineal sympathetically denervated animals. Night interruption by 1 h lighting (about 400 lux at the height of goat's head) resulted in an abrupt decline of CSF melatonin to the basal level within 30 min and immediate recovery to the previous high level after reestablishment of the dark phase. The CSF/plasma ratio was 10.8-18.4 during the dark phase and 1.7-1.8 during the light phase. CSF and plasma melatonin levels were also examined after exogenous melatonin given either peripherally or intraventricularly. Continuous subcutaneous infusion of melatonin (5 micrograms/h) maintained melatonin levels in the plasma about 3 times higher than that in the CSF during its application. On the other hand, an intraventricular injection of 2 micrograms melatonin elevated plasma melatonin by 100 pg/ml within 1 min. These results indicate that turnover of CSF melatonin is fairly rapid and favor a hypothesis for direct access of pineal melatonin to the brain ventricular system in the goat.

Melatonin in the extracellular space of the pineal gland was measured continuously for 4 consecutive days from single, freely moving rats by means of in vivo microdialysis. A robust circadian rhythm was observed in the pineal extracellular melatonin under both light-dark (LD) and continuous dark (DD) conditions, the patterns of which were almost identical for 4 days within individuals but varied substantially among individuals. The offset phase of melatonin rhythm was more stable than the onset phase. Light-induced phase shift of melatonin rhythm was measured in individual rats, which had been entrained to LD and subsequently released into DD. On the 1st day in DD, a 3-min light pulse of 200 lx was applied either at circadian time (CT) of 17 or 22 h (5 and 10 h after the dark onset, respectively). The light pulse rapidly suppressed the nocturnal melatonin level. The rate as well as the level of melatonin suppression was significantly greater by the pulse at CT22 than at CT17. A phase shift of the melatonin rhythm was calculated on the 2nd and 3rd days in DD. Significant phase delay shift was observed after the pulse at CT17 and advance shift after the pulse at CT22 of approximately 1 h in either case. Because the amount of phase shift was not different between the 2nd and 3rd days in DD, the phase shift of pineal melatonin rhythm by single light pulse seems to be completed immediately.

No abstract

Abstract. Meishan pigs are known for their early sexual maturity. On the other hand, they grow slowly. There is no information currently available about the combination of these two characteristics in Meishan pigs. To study the developmental characteristics of Meishan pigs, the plasma concentrations of LH, FSH, inhibin, testosterone, estradiol-17β, progesterone and insulin-like growth factor-I (IGF-I) in young Meishan boars were determined using RIA and ELISA. Inhibin decreased with age in weeks, while testosterone and estradiol-17β increased. Testosterone increased gradually, and an increase in estradiol-17β occurred after sexual maturity. IGF-I increased before puberty and subsequently decreased just after puberty like a pubertal IGF-1 surge. FSH, LH and progesterone did not change with age. There was no significant correlation among the hormones. During the experimental period, the Meishan boars showed large individual differences. These differences may depend on the fact that Meishan boar reach maturity at 12 weeks of age and continue to grow thereafter.