Under the condition of this study, irrigation method during root-canal treatment influenced coronal microleakage. NaOCl + EDTA and chlorhexidine gel allowed better sealing following root filling.

All the thermally treated systems resulted in similar apical transportation. In the cervical region, the Hyflex CM and Prodesign Logic systems were associated with more centred preparations.

Neither single- nor multiple-visit root canal treatment ex vivo, eliminated E. faecalis completely from dentinal tubules. Up to 60 days after root filling, E. faecalis remained viable inside dentinal tubules. When no sealer was used, E. faecalis presented a higher growth rate.