Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3/H4. The mechanism of survival and recovery of chromatin structure remains obscure. Here we show how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome was conducted. When Pol II approaches the area of strong DNA-histone interactions, a small intranucleosomal DNA loop (zero-size or Ø-loop) containing transcribing enzyme is formed. During formation of the Ø-loop, the recovery of DNA-histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3/H4 histones from DNA during enzyme passage.

Small DNA loops formed on transcribed nucleosomes trigger transcriptional arrest on damaged DNA and reveal hidden DNA breaks.

Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contains a pair of fluorescent labels (Cy3 and Cy5) allowing measuring relative distances between the neighboring coils of nucleosomal DNA using Forster resonance energy transfer (FRET). The single-particle FRET (spFRET) approach for analysis of DNA uncoiling from the histone octamer during transcription through chromatin is described in detail.

Aerogels with a density of 4.2−22.8 kg/m 3 were obtained from bacterial cellulose synthesized under static and dynamic cultivation conditions on a molasses medium. The strength properties and porous structure of the aerogels strongly depended on their density. With an aerogel density of 22.8 kg/m 3 , the modulus of elasticity at 80% compression of the sample was 0.1 MPa. The decrease in the density of aerogels led to an increase in the pore sizes ranging from 20 to 1000 μm and a decrease in the modulus of elasticity. These characteristics were more pronounced in aerogels obtained from bacterial cellulose under static cultivation conditions. The aerogels had a low coefficient of thermal conductivity (0.0257 W m −1 °C−1 ), which is comparable to the thermal conductivity of air, and moderate thermal stability because the degradation processes of the aerogels began at 237 °C. The aerogels obtained from bacterial cellulose had high sound absorption coefficients in the frequency range of 200−5000 Hz, which makes it possible to use the aerogels as heat-and sound-insulating materials.