According to decision no. 491/QD-TTg signed in 2018 by the Vietnamese Prime Minister approving adjustments to the national strategy for the general management of solid waste until 2025 with a vision toward 2050, Vietnam has committed itself to move toward collecting, transporting, and treating 100% of non-household waste by 2025 and 85% of waste discharged by households by 2025. This paper aims to determine which is the best sustainable solid waste management system out of those that have been formulated by World Bank experts for Hanoi until 2030 for implementing the national strategy. The paper compares four distinct solid waste management enhancement alternatives, namely, “Improving the current system for waste collection and transportation”; “Reducing, reusing, and recycling waste at source”; “Mechanical–biological treatment (MBT) plants for classifying, composting, and refuse-derived fuel (RDF) for the cement industry”; and “MBT plants for classifying, composting, and RDF for waste-to-energy/incineration plants”. The comparison was made using an analytic hierarchy process. As a result, the research indicated that “MBT plants for classifying, composting, and RDF for waste-to-energy/incineration plants” has the highest ranking in terms of a sustainable solution for the municipal solid waste management system. Therefore, it should be applied for managing the current situation in Hanoi. At the same time, the sustainable development of the system must seek to decrease the waste-to-energy ratio continuously and significantly through the planned reuse of materials that can be recycled to industry. According to the literature, in major cities in Asia and Africa, development programs are moving toward waste-to-energy solutions. The EU’s circular innovation programs and action plan may be in the opposite direction to this trend.
A uridine diphosphate-glucose:sterol glycosyltransferase-encoding gene was isolated and cloned from the established fosmid library of Micromonospora rhodorangea ATCC 27932 that usually produces the aminoglycoside antibiotic geneticin. The gene consists of 1,185 base pairs and encodes a 41.4 kDa protein, which was heterologously expressed in Escherichia coli BL21(DE3). In silico analyses of the deduced gene product suggested that it is a member of the family 1 glycosyltransferases. The recombinant protein MrSGT was able to catalyze the transfer of a glucosyl moiety onto the C-3 hydroxy function in sterols (β-sitosterol, campesterol, and cholesterol), resulting in the corresponding steryl glucosides (β-sitosterol-3-O-β-D-glucoside, campesterol-3-O-β-D-glucoside, and cholesterol-3-O-β-D-glucoside). This enzyme prefers phytosterols to cholesterol, and also shows substrate flexibility to some extent, in that it could recognize a number of acceptor substrates.
In this study, an efficient high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-ion trap-tandem mass spectrometry (MS/MS) was developed for the identification of the biosynthetic congeners involved in the aminocyclitol aminoglycosidic fortimicin pathway from Micromonospora olivasterospora fermentation. The usage of both acid extraction (pH ∼2.5) followed by an cationic-exchanging SPE cleanup and pentafluoropropionic acid mediated ion-pairing chromatography with ESI-ion trap-MS/MS detection was determined to be sufficiently practical to profile the fortimicin (FOR) congeners produced in a culture broth. The limit of the quantification for the fortimicin A (FOR-A) standard spiked in the culture broth was ∼1.6 ng mL(-1). The average recovery rate was 93.6%, and the intra- and inter-day precisions were <5% with accuracy in the range from 87.1 to 94.2%. Moreover, the epimeric mixtures including FOR-KH, FOR-KR, and FOR-B were separately resolved through a macrocyclic glycopeptide (teicoplanin)-bonded chiral column. As a result, ten natural FOR pseudodisaccharide analogs were identified and semi-quantified in descending order as follows: FOR-A, FOR-B, DCM, FOR-KH plus FOR-KR, FOR-KK1, FOR-AP, FOR-KL1, FOR-AO, and FOR-FU-10. This is the first report on both the simultaneous characterization of diverse structurally closely related FORs derived from bacterial fermentation using HPLC-ESI-ion trap-MS/MS analysis and the chromatographic separation of the three FOR epimers.
A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A , istamycin B , istamycin B , istamycin A , istamycin C, istamycin A , istamycin C , istamycin C , istamycin X , istamycin A , istamycin Y , istamycin B , and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
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