We have used our recently characterized human 3β‐hydroxy‐5‐ene steroid dehydrogenase/Δ5‐Δ4‐isomerase (3β‐HSD) cDNA as probe to isolate cDNAs encoding bovine 3β‐HSD from a bovine ovary λgtll cDNA library. Nucleotide sequence analysis of two overlapping cDNA clones of 1362 bp and 1536 bp in length predicts a protein of 372 amino acids with a calculated molecular mass of 42093 (excluding the first Met). The deduced amino acid sequence of bovine 3β‐HSD displays 79% homology with human 3β‐HSD while the nucleotide sequence of the coding region shares 82% interspecies similarity. Hybridization of cloned cDNAs to bovine ovary poly(A)+ RNA shows the presence of an approximately 1.7 kb mRNA species.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.