The effect of recombinant TNF-α on programmed death of donor lymphocytes was studied in vitro. The proapoptotic effect of this cytokine is realized through transcription factors and cell cycle inhibitors. Incubation of lymphocytes with recombinant TNF-α revealed increased levels of NF-kB and p21 and reducted content of nonphosphorylated p53.
We studied in vitro effects of recombinant interleukin-5, interleukin-3, and eotaxin on programmed death of eosinophils from healthy donors and patients with non-Hodgkin's lymphomas associated with severe blood eosinophilia. Interleukin-5 and eotaxin produced the most potent antiapoptotic effect on eosinophils from healthy donors. In patients with non-Hodgkin's lymphomas, spontaneous apoptosis in eosinophilic leukocytes was low and remained unchanged during incubation with recombinant proteins.
General patterns and peculiarities of changes in structure and function of red blood cell’s membrane during pathology of different genesis are considered in the article. Based on literature data and our own investigations one concludes concerning presence of typical molecular disturbances of erythrocyte’s membrane in different diseases. But general character of molecular modification of plasmatic membrane during pathology of different genesis is controversial. Universal mechanisms of structural and functional modification of structure and function of cells’ membrane are considered in details.
Characteristics of changes in the membrane potential induced by increased Ca ~-~ inward current in patients with lung cancer are presented. Both the amplitude and the rate of hyperpolarization induced by opening of Ca2+-activated potassium channels are markedly decreased in erythrocytes from patients in comparison with healthy donors. Under conditions of moderate Ca 2+ inward current, hyperpolarization is followed by restoration of the membrane potential. This process is more rapid in cancer patients. It is shown that the parameters of hyperpolarization in their dynamics during antitumor therapy depend on the type of lung cancer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.