Humic waters (HW) are globally unique, deep underground, dark-brown waters containing humic acids, and they present numerous therapeutic activities including anti-inflammatory. In the present study, we use HW from source in Poland. Diabetes has become an epidemic and is a risk factor of cardiovascular diseases. Hyperglycemia in diabetes is responsible for damaging of the endothelium and increases inflammation on the surface of the vascular lining. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6), and with the reduction of cell proliferation. In the study, we used cultures of endothelial cells (HUVEC line—human umbilical vein endothelial cells) with the addition 30 mM/L of glucose in the culture medium which imitated the conditions of uncontrolled diabetes. The addition of HW in the proper volume to the culture medium causes reduction of inflammation by significant decrease in inflammatory cytokines such as TNFα and IL-6 and also leads to enhancement of the cell proliferation. It appears that the adverse effects of hyperglycemia on vascular endothelial cells may be corrected by addition of humic water. The above promising results of in vitro tests provide an opportunity to the possible use of humic water in the supportive treatment of endothelial dysfunction disorders in diabetes. However, this issue requires further clinical research.

No abstract

In balneotherapy we normally use simple raw-peloidy or water for procedures. Several years of ourbalneochemistry researches indicate, that combination of certain natural resources could be more therapeutically effective than simple product. It is necessary, however, to choose proper compounds which are not influenced destructively on each others but work synergistically. In our opinion the following combination of natural healing resources occurring in Poland are possible:• the organic peloid (peat-borowina) with sulfide mineral water or with alkaline bicarbonate water • Inorganic peloid (Gytium) with brine or sulfide mineral water • combination two mineral waters: sulfide waters with natural humic waters or humic water with low mineralization brine Results: The combination of organic peloid with sulfide water (instead of water) intensified the healing properties of new product and made stabilization of H2S. When we use the low mineralization alkaline water to peloid the significant increased the amount of humic acids in the pulp peat was observed. On the basis of own research we excluded destructive action of sulphur compounds on the humic acids. The newly formed product resulting from a combination of peat and mineral waters can be used for wraps, bath of disperse or tampons.The combination of Gytium with brine water reduced the H2S smell from the new product. Hence, no irritant of H2S on the respiratory tract was observed Our next study showed that combination sulfide water with humic acid waters are very beneficiated. Both -sulfur( II) and humic acids were active in a new product and work synergistically. New product can be used for bathing and irrigation of body cavities.Conclusion. It is beneficial to combine peloids with selected healing waters and combine two mineral waters, but it is necessary perform balneochemical research before application. The research should prove that components included in the new products work synergistically but not destructively.

Introduction: Diabetes has become an epidemic and is a risk factor of cardiovascular disease. Hyperglycemia in diabetes is responsible for damaging of the endothelium and increases inflammation on the surface of the vascular lining. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) and by a reduction of cell proliferation.Humus waters (HW) containing humus acids with sources in Poland are originally bacteriologically pure, physically and chemically stable. Because of the physicochemical properties of humus acids of adsorption, complexation and ion exchange they present numerous therapeutic activities including anti-inflammatory. Data regarding its molecular mechanisms underlying these phenomena are scarce. The present study reveals that humus water exerts important beneficial effects on endothelial cells under conditions of hyperglycemia.Material and methods: Endothelial cells (HUVEC line) were derived from human umbilical veins using the enzymatic method with collagenase. Cells were cultured in M199 media in accordance the standard method. The experiment was repeated three times with three independent cells isolations. The study was conducted in four groups: 1-control group -culture medium without impact of glucose and humus water; 2 -an appropriate volume of humus water was added to obtain its 1% solution in the culture medium; 3-(30 mM/L) glucose added to the culture medium to imitate hyperglycemic condition; 4 -glucose, humus water in the medium. At the end of experiment, conditioned medium from each well of culture plates was collected and the concentration of TNF-α and IL-6 in the supernatant was measured by ELISA test (Diaclone) according to the manufacturer's instructions.

Chronic foot and leg ulcers are a serious medical problem requiring long-term treatment, often complicated by amputations and long-term disability. Balneological and physical methods complement the treatment of those patients who have completed optimal vascular and surgical treatment. The most common causes of ulcers are atherosclerosis, diabetes and venous insufficiency. In the thermal treatment we can effectively improve the treatment results using natural gases such as carbon dioxide and ozone, physical methods, kinesitherapy and treatment of diseases causing ulceration.The aim of our research was to evaluate the results of treatment of ulcers in patients with diabetes, atherosclerosis and venous insufficiency in the thermal center conditions.The material is a group of 46 patients with ulcers, in this group there were 18 women, the mean age was 63.5 years ± 9.8. The number of patients with diabetic, atherosclerotic and venous insufficiency type of ulcer was 56.5 and 17.4 had grade II, and another 3/4/5 respectively 23.2 / 14.2%. Treatment in addition to standard surgical care and antibiotic therapy included ozone-therapy, carbodioxide-therapy, kinesitherapy and physical treatments (pulsed magnetic field and laser therapy). The average duration of treatment was 46 days. The results of the treatment were assessed on a four-level scale: I complete recovery, II improvement with chance to full recovery, III minor improvement, and IV lack of the improvement. In addition, patients were evaluated for changes in metabolic control of diabetes, and lipid parameters were also evaluated.Full recovery was achieved in 32.9 improvement in the prognosis of recovery, small improvement in 24,6 of patients. In the subjective evaluation questionnaire, all patients reported improvement -generally reducing pain and swelling. In additional studies, the statistically significant level of total cholesterol, LDL-cholesterol, triglycerides and fibrinogen decreased.