In tro duc tion: Tour niquet due ve nous sta sis can al ter bo th con cen tra tion and/or ac ti vi ty of se ve ral blood ana lytes, but is ra re ly re gar ded as an is sue of la bo ra to ry va ria bi li ty. To over co me the prob lem tran sil lu mi na tion de vi ces (TD) ha ve been pro po sed for a sta si s-free phle bo to my. In this pa per the use of a TD in pla ce of tour niquet du ri ng blood col lec tion has been eva lua ted. Ma te ria ls and met ho ds: Blood was col lec ted from 250 vo lun tee rs di vi ded in fi ve ho mo ge nous grou ps of tour niquet ti mes (G1: 30 sec, G2: 60 sec, G3: 90 sec, G4: 120 sec, G5: 180 sec) and com pa red to blood ob tai ned usi ng TD. All sam ples we re ana lyzed for glu co se (GLU), total pro tein (TP), albumin (ALB), trig lyce ri des (TRIG), potas sium (K), sodium (NA), phos pha te (PHOS), cal cium (CA), alka li ne phos pha ta se (ALKP) and mag ne sium (MG). Re sul ts: In res pe ct of TD, G1 did not show sta tis ti cal ly sig ni fi ca nt in crea ses in all cli ni cal che mis try tes ts; G2 showed in crea ses for GLU, TP, ALB, TRIG, K, CA, MG and ALKP. G3 and G4, showed no sig ni fi ca nt in crea se on ly for PHOS. G5 showed sig ni fi ca nt in crea ses in all the tes ts eva lua ted. Mo reo ver, cli ni cal ly sig ni fi ca nt va ria tio ns we re ob ser ved for TP, ALB, K and CA in G2 to G5; for NA in G3 to G5; for MG in G4 and G5; for GLU, TRIG, ALKP on ly in G5. Con clu sio ns:The se re sul ts sup po rt the ap pli ca tion of TD in blood col lec tion for rou ti ne cli ni cal che mis try la bo ra to ry tes ts, sug ges ti ng its use shou ld be mo re diff u sed. Key wor ds: phle bo to my and blood col lec tion; pre ana lyti cal pha se; qua li ty ma na ge me nt system in cli ni cal la bo ra to ry; tran sil lu mi na tion; ve nous sta sis.
Recent years have witnessed a substantial increase in the number of seric determinations of vitamin D, in a worldwide basis. At Hospital das Clínicas of Faculdade de Medicina of Universidade de São Paulo that increase reached 700% over the last four years. Nevertheless there are many controversies on the literature about the role of vitamin D in conditions unrelated to the musculoskeletal system. In this study the metabolism, sources and actions of vitamin D on the body are reviewed. Observational studies, clinical trials, systematic reviews and metanalysis which focused on the relationship between the vitamin and conditions such as cancer, cardiovascular disease, diabetes and falls were searched on the literature, analyzed and discussed. Results are presented as quiz and answer, tables and a figure. The role of vitamin D on the above-mentioned conditions is discussed, and the controversial issues stressed.
During the 3-month period that our 23 cystinosis patients were treated with NAC, oxidative stress was reduced and renal function significantly improved. No side-effects were detected. Larger and controlled studies are needed to confirm these findings.
Background. Health and social management of the SARS-CoV-2 epidemic, responsible for the COVID-19 disease, requires both screening tools and diagnostic procedures. Reliable screening tests aim at identifying (truely) infectious individuals that can spread the viral infection and therefore are essential for tracing and harnessing the epidemic diffusion. Instead, diagnostic tests should supplement clinical and radiological findings, thus helping in establishing the diagnosis. Several analytical assays, mostly using RT-PCR-based technologies, have become commercially available for healthcare workers and clinical laboratories. However, such tests showed some critical limitations, given that a relevant number of both false-positive and false-negative cases have been so far reported. Moreover, those analytical techniques demonstrated to be significantly influenced by pre-analytical biases, while the sensitivity showed a dramatic time dependency. Aim. Herein, we critically investigate limits and perspectives of currently available RT-PCR techniques, especially when referring to the required performances in providing reliable epidemiological and clinical information. Key Concepts. Current data cast doubt on the use of RT-PCR swabs as a screening procedure for tracing the evolution of the current SARS-COV-2 pandemic. Indeed, the huge number of both false-positive and false-negative results deprives the trustworthiness of decision making based on those data. Therefore, we should refine current available analytical tests to quickly identify individuals able to really transmit the virus, with the aim to control and prevent large outbreaks.
Introdução:A cistatina C sérica tem sido apontada como um marcador de filtração glomerular. Objetivo: Realizar a validação de um método específico e automatizado, a imunonefelometria, mensurando os níveis séricos de cistatina C por meio do nefelômetro da empresa Behring (BN II) e correlacionar resultados obtidos entre pacientes transplantados. O ensaio perfaz o intervalo de referência de 0,23-7,25 mg/l. A imprecisão intra e interensaio foi de 8,73% e 5,38%, respectivamente. A recuperação analítica da cistatina C após adição de controle foi entre 86,7 % e 98% (média 92,3%). A estabilidade da cistatina C à temperatura ambiente, sob refrigeração e sob congelamento foi testada. A perda mais significativa foi encontrada nas amostras armazenadas à temperatura ambiente, em que foram perdidos até 10% da concentração inicial. Foi encontrado coeficiente de variação de 14,79% para sensibilidade analítica. Durante todo o processo foram comparados os resultados com o controle de qualidade e obtivemos bons resultados. Depois desses testes, nós comparamos as correlações em três grupos de pacientes transplantados renais sob diferentes esquemas de imunossupressão (n = 197) -azatioprina (n = 36), micofenolato mofetil (n = 131) e sirolimus (n = 30) -entre as equações de estimativa de filtração glomerular (Cockroft Gault, Nankivell e Modification of Diet in Renal Disease) e cistatina C sérica ou creatinina sérica. Conclusão: O ensaio nefelométrico cistatina C pode perfeitamente ser adequado à nossa rotina laboratorial e as correlações entre creatinina sérica e as diferentes equações de estimativa de filtração glomerular são melhores do que quando comparamos as mesmas à cistatina C nos três grupos, independentemente da terapia imunossupressora utilizada. resumo unitermos Cistatina Nefelometria Função renal Transplante Imunossupressores abstractIntroduction: Serum cystatin C has been identified as a glomerular filtration marker. Objective: To validate immunonephelometry, a specific and automated method, by measuring levels of serum cystatin C through Behring nephelometer (BN II) and correlate results among transplant patients. The assay comprises the reference range of 0:23 to 7:25 mg/l. The intra-assay and inter-assay imprecision rates were 8.73% and 5.38%, respectively. The analytical recovery of cystatin C after addition of control was between 86.7% and 98% (average 92.3%). The stability of cystatin C to room temperature, refrigerated or frozen was tested. The most significant loss was found in samples stored at room temperature, in which up to 10% of the initial concentration was lost. The coefficient of variation was 14.79% for analytical sensitivity. Throughout the process the results were compared with quality control and good results were achieved. After these tests, we compared the correlations between equations for estimating glomerular filtration rate (Cockroft Gault, Nankivell and MDRD) and serum cystatin C or serum creatinine in three groups of kidney transplant patients under different immunosuppressive regimens (n = 197...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.