system at which we were able to target attempts at improvements. We would like to point out that we have a policy for telephoning all important results to the clinicians directly, so by-passing the written report.In future, the computerisation of the microbiology service with terminals in all clinical areas will lead to a more efficient turnaround time. selected patients whose blood and bone marrow were cultured for S typhi to determine the yield of S typhi from these two sites. MethodsBlood and bone marrow samples were collected from 100 patients who had fever of unknown origin. Each sample was collected in two blood culture bottles containing brain heart infusion and thioglycollate broth, respectively. About 5 ml of venous blood was inoculated in each bottle containing 45 ml of broth; similarly, 0-5 ml-1 0 ml of bone marrow was collected in each bottle containing 45 ml of broth. Bottles were sent immediately to the laboratory and incubated at 37°C for seven days. Each bottle was examined daily and subcultured on to blood agar and MacConkey's media after 24, 48, and 72 hours, and on the seventh day of incubation.Non-lactosing fermenting colonies from MacConkey's medium were tested for S typhi by slide agglutination test with specific antisera.56 Biochemical tests were performed by using API 20E strips. ResultsThe samples of blood and bone marrow were processed at The Aga Khan University Hospital's diagnostic laboratory. S typhi was isolated from the blood (n = 58) or bone marrow (n = 88). The blood and bone marrow cultures of 12 patients showed no growth for any bacteria.Thirty blood samples from 88 patients (whose bone marrow were positive) showed no bacterial growth.
Background Diabetes mellitus is a chronic disease characterized by hyperglycemia that may occur due to genetic, environmental or lifestyle factors. Natural remedies have been used to treat diabetes since long and many antidiabetic compounds of varied efficacies have been isolated from medicinal plants. Rhazya stricta has been used for decades for the treatment of diabetes mellitus and associated ailments. Considering the folkloric use of R. stricta against diabetes, it was aimed to investigate the effectiveness of its root extracts against diabetes through in vitro assays and in vivo studies using animal model along with phytochemical profiling through GCMS. Methods Various fractions of Rhazya stricta obtained through column chromatography were evaluated for a variety of assays including α-glucosidase, Dipeptidyl peptidase-IV (DPP-IV), β-secretase and Glucagon-like peptide-1 (GLP-1) secretion studies. For the in vivo studies the alloxan-induced diabetic mice were treated with root extracts and blood glucose levels, HbA1C, and other biochemical markers along with the histological study of the liver were done. The phytochemical identification was performed using an Agilent 7890B GC coupled to a 7010 Triple Quadrupole (MS/MS) system. GraphPad Prism software version 5.01 was used for statistical analysis. Results Majority of the extract fractions showed excellent results against diabetes by inhibiting enzymes DPP-IV (Up to 61%) and β-secretase (Up to 83%) with IC50s 979 μg/ml and 169 μg/ml respectively with increase in the GLP1 secretion. The results of in vivo studies indicated a marked reduction in blood glucose and HbA1c levels along with positive effects on other parameters like lipid profile, liver functions and renal functions of extract-treated mice as compared to control. The histological examination of the liver demonstrated hepatoprotective effects against diabetes led changes and various classes of phytochemicals were also identified through GCMS in different fractions. Conclusion The results revealed strong antidiabetic activity of R. stricta root with the potential to protect body organs against diabetic changes. Moreover, a variety of phytochemicals has also been identified through GCMS that might be responsible for the antidiabetic potential of Rhazya stricta root. Graphical abstract
In the present study anatomical characterization of 30 species of Fabaceae endemic to Lahore, Pakistan were done under light and scanning electron microscopy. Variety of qualitative and quantitative anatomical characters like epidermal cells shapes and size, stomata types, length, and width of guard cells, subsidiary cells, trichomes, silica bodies, shapes, and their numbers were studied. Overall polygonal, irregular smooth, thick walled epidermal cells were observed at both abaxial and adaxial surfaces except Dalbergia sisso Roxb in which hexagonal epidermal cells were reported. Milletia ovelifolia Kurz. possessed the largest length of epidermal cell i.e., 273.1 μm whereas Calliandra bella Benth. showed the smallest length i.e., 76.5 μm. Average width of epidermal cells ranged from 44 to 265.5 μm. M. ovelifolia Kurz had largest width while Acacia nilotica L. had the smallest width respectively. In adaxial surface epidermal cells length ranged 317 to 46.4 μm, Glycyrriza glabra L. showed the smallest length whereas Prosopis juliflora DC. had largest length. In adaxial numbers of stomata is high as compare to the abaxial surface, mostly paracytic, anisocytic, and anomocytic stomata were observed. There is not much variations observed in trichomes of studied members. Generally non glandular, unbranched, uniserate, mulicellular bulbous base with pointed tips were reported. Oval, rounded, triangular shaped silica bodies were observed in some species. It is concluded that qualitative and quantitative anatomical variations in epidermal cells, stomata and trichomes are of good taxonomic value for the studied Fabaceae species.
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