Salinity is one of the major environmental factors limiting growth and productivity of rice plants. In this study, the effect of salt stress on phospholipid signaling responses in rice leaves was investigated. Leaf cuts were radiolabeled with 32P-orthophosphate and the lipids extracted and analyzed by thin-layer chromatography, autoradiography and phosphoimaging. Phospholipids were identified by co-migration of known standards. Results showed that 32Pi was rapidly incorporated into the minor lipids, phos-phatidylinositol bisphosphate (PIP2) and phosphatidic acid (PA) and, interestingly, also into the structural lipids phosphatidylethanolamine (PE) and phosphatidylglycerol (PG), which normally label relatively slowly, like phosphatidylcholine (PC) and phosphatidylinositol (PI). Only very small amounts of PIP2 were found. However, in response to salt stress (NaCl), PIP2 levels rapidly (<30 min) increased up to 4-fold, in a time- and dose-dependent manner. PA and its phosphorylated product, diacylglyc-erolpyrophosphate (DGPP), also increased upon NaCl stress, while cardiolipin (CL) levels decreased. All other phospholipid levels remained unchanged. PA signaling can be generated via the combined action of phospholipase C (PLC) and diacylglycerol kinase (DGK) or directly via phospholipase D (PLD). The latter can be measured in vivo, using a transphosphatidylation assay. Interestingly, these measurements revealed that salt stress inhibited PLD activity, indicating that the salt stress-induced PA response was not due to PLD activity. Comparison of the 32P-lipid responses in salt-tolerant and salt-sensitive cultivars revealed no significant differences. Together these results show that salt stress rapidly activates several lipid responses in rice leaves but that these responses do not explain the difference in salt tolerance between sensitive and tolerant cultivars.
Detailed subsurface studies of the northern part of the Suez Rift and adjacent areas indicate the superposition of two different episodes of deformation. During the earlier (Late Cretaceous) phase of deformation, folds with NE‐SW oriented axes were formed in northern Egypt as a result of convergence between Africa and Eurasia and the closure of the Neotethys. During the later (early Miocene) deformation., NW‐oriented normal faults were formed as a result of the opening of the Suez Rift. Borehole data have shown that a belt of NE en échelon folds with NE‐SW axes exists in the subsurface in the northernmost part of the rift, between Ayun Musa and the Sukhna‐1 well, soulth of Geble Ataqa. This fold belt represents the SW continuation of the en échelon folds exposed in the Mitla Pass, to the NE of the rift. Another pre‐rift structure is the offshore extension of the Wadi Araba structure as a SE‐facing monocline. This offshore structure also represents the continuation of the Geble Somar structure, on the eastern shoulder of the Suez Rift. The Geble Somar and Wadi Araba Structures represent the southernmost prerift folds in northern Egypt. Pre‐rift folds in the study area stood high above sea‐level during the Palaeocene and early Eocene. Upper Cretaceous and/or older rocks in the cores of these folds were later unconformably covered by middle Eocene rocks. The presence of Late Cretaceous folds should be taken into consideration when exploring for hydrocarbons in this part of the Suez Rift. Borehole data in NE Egypt also indicate the presence of Late Cretaceous folds underneath the almost flat‐laying Tertiary rocks in the northern part of the Eastern Desert. These folds are condidered to be potential hydrocarbon traps in this relatively poorly‐explored area.
Over the last few years, Saint Katherine, South Sinai has seen large scale development in connection with building new cities, land reclamation, and tourism. The Wadi El-Sheikh aquifer constitutes one of the major freshwater recourses in the Saint Katherine area. In that regard, ten Schlumberger vertical electrical soundings (maximum [Formula: see text]) associated with a number of measured and∕or calculated geoelectric and petrophysical parameters of the aquifer were conducted to delineate and assess the aquifer. Furthermore, subsurface stratigraphic data and measured hydrological parameters of shallow boreholes in the study area were integrated with the geoelectrical results. The integration of the sounding results, borehole data, and geoelectric and petrophysical parameters effectively delineated the alluvial fresh water aquifer with true resistivities ranging between 206 and [Formula: see text] and thickness ranging between 32 and [Formula: see text]. The northern part is characterized by a gradual decrease in porosity, electrical anisotropy coefficient, and total dissolved solid (TDS) concentrations. On the other hand, the northern part is characterized by a gradual increase in grain size, tortuosity, permeability, and transmissivity reflecting high aquifer potential. Moreover, the downward gravitational movement of groundwater is greater in the northern direction as well. Based on these findings, deep wide-spaced development water wells ([Formula: see text] depth) are recommended to be drilled in the northern area.
Nuweiba is one of Egypt’s strategic marine harbors and tourist sites located on the western coast of the Gulf of Aqaba. The absence of freshwater resources in Nuweiba necessitates investigations of local groundwater aquifers. Determination of the hydro-geophysical parameters of the Nuweiba alluvial fan constitutes the basic foundation for optimizing groundwater exploitation over time. Fifteen Schlumberger vertical electrical soundings (maximum [Formula: see text]), augmented by borehole stratigraphic data, aquifer tests, hydro-chemical analysis, and numbers of measured and/or calculated hydraulic parameters, were carried out in the study area. The fresh to brackish aquifer of Quaternary and pre-Quaternary alluvium sediments was effectively delineated with true resistivity and thickness ranges of [Formula: see text] and [Formula: see text], respectively. The western part of the aquifer has a large volume of fresh ground water for extraction and is characterized by a gradual increase in thickness [Formula: see text], grain size, transmissivity [Formula: see text], and a possible decrease in total dissolved solids levels [Formula: see text] and electrical anisotropy (1.10–1.11). Porosity (30.3–30.4%), tortuosity (1.348), formation factor (6) and hydraulic conductivity [Formula: see text] are essentially constant across the aquifer. The measured storativity of the aquifer is [Formula: see text], which is in accordance with the typical value of the unconfined aquifer at shallow depths.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.