Outbreaks of influenza D virus (IDV) continue to be reported in many countries. On the basis of the hemagglutinin-esterase fusion (HEF) gene, five IDV genetic lineages have been identified: D/OK, D/660, D/Yama2016, D/Yama2019 and D/CA2019 lineages. Previously reported IDV strains in China all form a sub-clade (D/China sub-lineage) within D/OK lineage. From October 2021 to February 2022, nasal swab samples (n = 250) were collected from apparently healthy cattle in slaughterhouses around the city of Guangzhou, China, and screened for IDV by RT-PCR. Ten samples were positive for IDV. An IDV strain with nearly complete genome sequences was identified and designated as D/bovine/CHN/JY3001/2021. Importantly, sequence alignments and phylogenetic analyses revealed that this IDV strain is genetically close to the strains (>98% homology) in the D/Yama2019 lineage that has been found only in Japan, but distant from the previously reported Chinese IDV strains (~95% similarity). These results demonstrate the emergence of D/Yama2019 lineage IDV in Chinese cattle herds, highlighting a need for future surveillance of D/Yama2019-like viruses toward better understanding both epidemiology and diversity of IDV in China.
Recent years have witnessed tremendous progress of intelligent robots brought about by mimicking human intelligence. However, current robots are still far from being able to handle multiple tasks in a dynamic environment as efficiently as humans. To cope with complexity and variability, further progress toward scalability and adaptability are essential for intelligent robots. Here, we report a brain-inspired robotic platform implemented by an unmanned bicycle that exhibits scalability of network scale, quantity and diversity to handle the changing needs of different scenarios. The platform adopts rich coding schemes and a trainable and scalable neural state machine, enabling flexible cooperation of hybrid networks. In addition, an embedded system is developed using a cross-paradigm neuromorphic chip to facilitate the implementation of diverse neural networks in spike or non-spike form. The platform achieved various real-time tasks concurrently in different real-world scenarios, providing a new pathway to enhance robots’ intelligence.
In the treatment of carcinoma of preclinical study, Gene products are delivered into tumor by a variety of routes which include intratumoral injection [1][2][3] , intraarterial gene delivery [4, 5] , intraperitoneal injection [6] and intravenous administration [7][8][9] , etc. However, because of different gene products and unit, it is difficult to evaluate gene transduction efficiency of these injection routes based on these studies. This study was designed to evaluation the p53 gene transduction efficiency and safe of rAd/p53 by intratumoral injection and intravenous administration. Animal experiments were performed in accordance with institutional guidelines and approved by the committee on the use and care on animal. Materials and methods Cell lines and cell cultureThe human colon carcinoma cell line SW480 was purchased from Shanghai cell bank of chinese academy of Sciences. The cells were cultured in RPMI 1640 with 10% fetal calf serum, 1000 U/mL penicillin, and 1mg/mL streptomycin The cells were grown at 37 in a humidifield atmosphere of 5% CO 2 for 3-4 days until they reached the logarithmic phase of growth. Then the cells were enzymatically detached using 0.25% trypsin-EDTA mixture for 20 min. Trypan blue exclusion was used to determine viable cell counts. Cells were prepared for subcutaneous inoculation by re-suspension in 1 × 10 7 cells/1 mL of DBPS (Dulbecco's phosphate buffered saline). Hepatic metastases model of colon cancerBALB/c nude mice were obtained from the animal center of Sun Yat-sen university. Two female nude mice (6 weeks of age) were inoculated subcutaneously with 0.5 × 10 7 SW480 cells of each one. Ten days after tumor cell inoculation, the tumors in the subcutaneous tissue were excised and divided into small tissues of 1-2 mm 3 . Laparotomy was performed on another anesthetized forty-six nude mice (5 to 6 weeks of ages, half female, half male), Abstract Objective: To evaluate transduction efficiency with recombinant adenovirus-mediated p53 (rAd/p53) therapy in a human colon cancer mouse model by intra-tumoral injection and intra-arterial delivery. Methods: The tumor pieces of human colon cancer SW480 were implanted in the livers of 45 nude mice. These mice were administrated with rAd/p53 by intratumoral injection and intra-arterial delivery. After 24 h, 48 h and 72 h rAd/p53 administration, 5 mice each group were killed with over anesthesia and their livers were removed. P53 expression and apoptosis of tumor and liver were assessed. Results: P53 expression and apoptosis of intratumoral administration group was higher than tail vein group and control group. Apoptosis and p53 expression of livers in three groups had no significant difference. Conclusion: p53 gene transduction efficiency and anticancer effect of rAd/p53 is much better by intra-tumoral injection than intra-arterial delivery.
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