This research was designed to evaluate the CNS depressant, anxiolytic, and analgesic action of aqueous and ethanol extract of Ganoderma applanatum , a valuable medicinal fungus used in multiple disorders belongs to Ganodermataceae family. Two extracts of G. applanatum were prepared using distilled water and ethanol as solvents and named AEGA and EEGA. Open field method, rotarod method, tail suspension method, and hole cross method were utilized for the CNS depressant action. In contrast, elevated plus-maze test and hole board method were utilized for the anxiolytic action. For determining the analgesic potential, acetic acid-induced writhing test, hot plate method, and tail immersion test were used. Besides, molecular docking has been implemented by using Discovery studio 2020, UCSF Chimera and PyRx autodock vina. At both doses (200 and 400 mg/kg) of AEGA and EEGA showed significant CNS depressant effect ( p < 0.05 to 0.001) against all four tests used for CNS depressant activity. Both doses of AEGA and EEGA exhibited important anxiolytic activity effect ( p < 0.05 to 0.001)against the EPM and hole board test. Both doses of AEGA and EEGA also exhibited a potential analgesic effect ( p < 0.05 to 0.001) against all three tests used for analgesic action. In addition, in the molecular docking the compounds obtained the scores of −5.2 to −12.8 kcal/mol. Ganoapplanin, sphaeropsidin D and cytosporone C showed the best binding affinity to the selected recptors. It can be concluded that AEGA and EEGA have potential CNS depressant, anxiolytic, and analgesic action, which can be used as a natural antidepressant, anxiolytic, and analgesic source.

biocompatible, economical and easily available, they are best likened to semi-synthetic excipients because of their less toxicity, low cost, availability, emollient and non-irritant nature. 5,6 The synthetic polymers have certain disadvantages, such as high cost, toxicity, environmental pollution during synthesis, non-renewable sources, side effects, less patient compliance, etc. 7 Tinospora cordifolia (Figure 1) commonly called heart-leaved moonseed in English, Guduchi or Giloy in Hindi and Gulancha in Bengali belongs to the family Menispermaceae. It is a genetically disparate, large, deciduous climbing shrub with greenish yellow typical flowers, found at higher altitude. 8-10 Recently, the plant is of great interest to researchers across the world due to its medicinal properties like anti-diabetic, anti-inflammatory, anti-oxidant, antiallergic, anti-malarial, anti-periodic, anti-spasmodic, anti-arthritic, antistress, immunomodulatory, hepatoprotective and anti-neoplastic activities. 11 In the present study, an extraction was made for mucilage from the stems of T. cordifolia and investigated for the possibility of use as binder and release modifying material in the formulation of drug in solid dosage forms. Diclofenac sodium, a Non-steroidal Antiinflammatory Drug (NSAID) which is commonly used in the management of pain of various etiologies. 12 Materials and Methods

Gastro retentive drug delivery systems (GRDDS) are the systems which are retained in the stomach for a longer period of time and thereby, improve the bioavailability of drugs. The objective of this study was to formulate gastro retentive floating matrix tablet of Mebhydrolin Napadisylate using different ratios of HPMC K4M & HPMC K100LV. The sodium bi carbonate and citric acid was used in combination as gas generating agent. The tablets were prepared by wet granulation method and evaluated for the pre and post compression parameters such as angle of repose, tapped density, bulk density, Carr's Index, Hausner's ratio, hardness, friability, weight variation, thickness, swelling index, In vitro buoyancy studies and In vitro drug release studies. The tablets containing Mebhydrolin Napadisylate released 61.6 to 87.6% of drug at the end of 10 hrs by In vitro release study. Formulation F3 containing HPMC K4M and HPMC K100LV at a ratio of 1:1 found to be the best formulation in terms of drug release and In vitro buoyancy time. The drug release followed the Higuchi model controlled mechanism of tablet Mebhydrolin Napadisylate. FTIR spectrums obtained suggest that there was no significant chemical interaction between the polymers and drug.

Aims: Lablab purpureus (Fabaceae) a well-known Bangladeshi vegetable combines a great number of qualities with its adaptability and functional food properties. But still there is no evidance based report on neuropharmacological activities of Lablab purpureus seeds. For this reason, the present study was designed to analyze in vivo CNS effect of methanolic extract of Lablab purpureus seeds. Place and Duration of Study: Department of Pharmacy, USTC between April 2017 and May 2018. Methodology: In this study, the crude seeds extract of Lablab purpureus was evaluated for its sedative and anxiolytic effect using animal behavioral models, such as open field and hole cross tests for its sedative properties and an elevated plus maze (EPM) test for its anxiolytic potential. Results: In open field and hole cross test, the seeds extract of Lablab purpureus showed a dose dependent suppression of motor activity and exploratory behaviour. In EPM test, animals treated with higher dose of methanolic extract showed significant (p<0.01) increased exploration to and time spent in EPM open arms. Conclusion: This study provide in vivo evidence that the methanolic extract of Lablab purpureus has significant sedative and anxiolytic activity. Further studies on active constituent of the extract can provide approaches for therapeutic intervention.

Aims: Tinospora cordifolia is a herb used in the traditional folk medicine due to their health benefits. Studies reported the biochemical composition and some biological properties of the plant stem. The aim of the current study was to investigate the antimicrobial and cytotoxic properties from the extract of Tinospora cordifolia stem. Place and Duration of Study: The study was accomplished by eleven months in the Department of Pharmacy, University of Science and Technology Chittagong (USTC), Bangladesh.  Methodology: Presence of phytochemical constituents such as alkaloids, flavonoids and tannins, saponins were evaluated. Antimicrobial activity of the stem extract was assayed separately using an agar diffusion method against some gram negative and gram positive bacteria. The cytotoxic potential of the stem extract was evaluated by the Brine-Shrimp Lethality Assay mathod.  Results: The zone of inhibition formed by the methanolic extract in the antimicrobial assays was significant. In addition, cytotoxicity of the extract was found to be significant as compared to the standard gallic acid in Brine-Shrimp lethality assay. Conclusion: The methanolic extract of Tinospora cordifolia may possess significant antimicrobial property which requires more studies to isolate the specific bioactive compound for developing a new antimicrobial agent from this plant.