The ideal cell-carrier material for cartilage regeneration should be one that closely mimics the natural environment in a living articular cartilage matrix. In the current study, we considered that alginate-based chitosan hybrid biomaterials could provide excellent supports for chondrocyte adhesion. To test this hypothesis, we investigated the adhesion behavior of rabbit chondrocytes onto an alginate polymer versus the adhesion of the chondrocytes onto some alginate-based chitosan hybrid polymer fibers in vitro. We demonstrated that the alginate-based chitosan hybrid polymer fibers showed much improved adhesion capacity with chondrocytes in comparison with alginate polymer fiber. Additionally, morphologic studies revealed maintenance of the characteristic round morphology of the chondrocyte and the dense fiber of the type II collagen produced by the chondrocytes in the hybrid polymer. On the basis of these results, we conclude that an alginate-based chitosan hybrid polymer fiber has considerable potential as a desirable biomaterial for cartilage tissue scaffolds.

Shipboard and shore-based investigation on siliceous and calcareous microfossil biostratigraphy, magneto-stratigraphy and tephrostratigraphy identified numerous datum events from the sedimentary sequences of Sites 1150 and 1151 drilled on the forearc basin of northern Japan by the Ocean Drilling Program Leg 186. Some 83 datum events were selected to construct new age-depth models for the sites. Based on the reliable magnetostratigraphy from the Pleistocene to the Upper Miocene, which were correlated to the standard geomagnetic polarity timescale, and on excellent records of diatom and radiolarian biostratigraphy throughout the sequences, the shipboard age model was revised. Major revisions referred to stratigraphic position of the Miocene-Pliocene boundary that has been shifted more than 200 m downward in each sequence. The age-depth relations of the forearc sites represent drastic changes in the sedimentation rate-extremely high (40 cm/ k.y. on average) in the Early Pliocene and low (less than 2 cm/k.y. on average) in the Middle Miocene-and several hiatuses exist throughout the sequence. The drastic changes can be related mostly to changes in diatom sedimentation and the tectonics of the Japanese Island Arc. Local ages for some foraminiferal, calcareous nannofossil and radiolarian bioevents are estimated from the age-depth models at each site. These newly calibrated bioevents and biozones as well as established diatom biostratigraphy are incorporated into the updated magneto-biochronologic timescale, which will contribute to an improvement in biochronologic accuracy of Neogene sediments in northern Japan and adjacent areas.

The effect of calcium compounds [Ca(HCOO)2-A and Ca(NO3)2] on the incidence of Phytophthora stem rot of soybean (Glycine max) cv. Tanbakuro was investigated in the field. Disease incidence in control plants in three fields naturally infested with Phytophthora sojae ranged from 11.7 to 52.0% at 140 days after transplanting. Independent of the pathotype diversity, 4 and 10 mM of the calcium compounds applied twice (prior to transplanting and 14 days after transplanting) significantly suppressed disease incidence and delayed onset. Ca(HCOO)2-A (Suicaru) was more effective than calcium nitrate for reducing disease incidence. In most cases, the calcium amendments increased plant height, number of nodes and pods, and seed yields, and reduced low-quality seeds. Scanning electron microscopy with fresh samples showed increased accumulation of calcium crystals around the cambium and xylem elements of soybean plants treated with 10-mM Ca(HCOO)2-A and Ca(NO3)2. Mycelial penetration was inhibited at these sites. These results indicated that calcium-rich areas may be more resistant to invasion by P. sojae, and the calcium crystals may play an important role in calcium ion storage and its availability for those tissues to maintain long-term field resistance.

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