Methods to isolate fungi from single spores are outlined. These methods are specifically designed for mycological laboratories which are not necessarily well funded. Therefore, they involve a simple procedure, are relatively inexpensive, and most importantly effective. Furthermore, only basic equipment is required. By using these methods, most fungi, with the exception of those that do not germinate on artificial medium, can be isolated. Some approaches are suggested to prevent mite infestations and to reduce the risk of bacterial contamination.
Minnesota is the top sugar beet (Beta vulgaris L.) producing state in the United States. In 2020, sugar beet plants were observed for the first time in which the two to three oldest leaves had light brown to dark brown necrotic leaf lesions that eventually became yellow or brown and died but remained attached to the plant. Morphological data and sequences of internal transcribed spacer regions identified the pathogen as Sclerotinia sclerotiorum. Because over 90% of the plants in identified fields were infected it was difficult to quantify loss in yield or quality caused by this disease. All fields with symptomatic plants had soybean or edible beans in the rotation. One field planted to several different varieties indicated that all the varieties were symptomatic. It will be useful to determine any economic loss caused by S. sclerotiorum and any known varietal resistance to this pathogen.
Establishing an adequate plant population is one of the first challenges of sugar beet production. Reduced sugar beet emergence results in a decision between a lower than desired plant population or replanting the field. The objective of this study was to determine the plant population that warrants replanting a field to maximize extractable sugar ha -1 . The study was conducted in three environments during the 2016 and 2017 growing seasons. Two planting dates and six plant populations were utilized in each environment. The two planting dates were separated by 19 or 20 days to simulate a replant situation. Sugar beets in each planting date were hand thinned to six populations of 44
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