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The Neolithic expansion in the Iberian Peninsula is marked by the introduction of livestock and domesticated crops which modified subsistence strategies in an unprecedented manner. Bulk collagen stable carbon and nitrogen isotope analysis has been essential to track these changes, which have largely been discussed in relation to particular geographic areas or single case studies. This paper reviews the available isotope literature to provide a regional, long-term synthesis of dietary changes associated with the expansion of the Neolithic and the establishment of farming economy in the Iberian Peninsula. Bulk collagen stable carbon and nitrogen isotope values of 763 human individuals and 283 faunal remains from the Mesolithic to the Late Neolithic period in Iberia (ca. 8000-3000 cal BC) were collated and analysed using a Bayesian mixing model. The results show that Mesolithic diets were isotopically diverse in both the Atlantic and Mediterranean regions of the Iberian Peninsula, and that a significant decrease in variability happened with the Neolithisation, culminating with the establishment of farming economies and reliance on terrestrial resources in the Late Neolithic.

This palaeodietary study presents carbon and nitrogen stable isotope data from human and faunal skeletal remains from the Copper Age settlement of Valencina de la Concepción–Castilleja de Guzmán, located in Seville, Spain. Montelirio, the only Valencina–Castilleja human group from which we have obtained reliable palaeodietary results, had a diet based on C3 terrestrial resources, including both plants and animals. The protein component of the diet consisted mainly of meat, milk and dairy products from livestock as well as C3 plant protein from cereals and pulses. This study compares data from Montelirio, the Copper Age group from Valencina–Castilleja, with the published data from other Iberian Late Neolithic–Copper Age (LN‐CA) societies. This comparison reveals a homogeneous diet with some exceptions. Overall, the LN‐CA diet in the Iberian Peninsula consisted mainly of animal proteins from meat, milk and dairy products from livestock as well as C3 plant sources from the characteristic agriculture of the societies of these periods. This study also demonstrates the minor use of aquatic resources from the Neolithic to the Copper Age period in the Iberian Peninsula. Copyright © 2015 John Wiley & Sons, Ltd.

Stable isotope investigations of the Prehistory of the Western Mediterranean have increased exponentially during the last decade. This region has a high number of Mesolithic and Neolithic carbon and nitrogen isotope ratio data available compared with other world areas, resulting from the interest in the "transition" between hunter-gathering and farming. This type of analysis is important as one of the few tools that give direct information on the poorly understood dietary transition from hunter-gatherer to agro-pastoralist subsistence in the Mediterranean Basin. Carbon and nitrogen stable isotope analysis on bulk collagen are especially useful for exploring marine vs. terrestrial protein input and therefore assess marine resource exploitation by these two different lifestyles. Gathering together all isotopic data for these chronologies we show that the Western Mediterranean underwent a unique/distinct Neolithisation process. These data show a gradual dietary shift in aquatic resource consumption during the transition to farming that contrasts to elsewhere in Europe.

The reported variations in interpopulational subsistence strategies among the compared Mediterranean societies do not seem to be directly related to the settlement region. Together with archeological data, this indicates the influence of socioeconomic factors in the Neolithic human diet. A general tendency toward a lesser use of aquatic resources is seen in this period in Iberia and the rest of the Mediterranean, as also documented for contemporary communities in the west and north of Europe. The data obtained will be important for further studies of socioeconomic patterns in European Neolithic societies.