The ability of Diatomacious earth (DAE) in reducing the toxic effects of aflatoxin (AF) in broiler diet was evaluated. DAE was supplemented @ 400 and 800 mg kg -1 of feed along with 0.5 and 1 ppm of AF kg -1 of feed. Healthy unsexed day old broiler chicks (n=360) were assigned to 9 groups comprising of control and treatment groups. Feeding of AF resulted in significantly lower feed intake as well as body weight gain and increase in feed conversion ratio in comparison to the control groups. Supplementation of DAE in aflatoxin mixed diet significantly reduced the deleterious effects of AF on growth parameters. Feeding of AF alone caused significant decrease in serum levels of total proteins, albumin, triglycerides and cholesterol. However, significant increase was found in serum levels of AST, ALT, ALP, BUN and creatinine in AF fed broilers. Supplementation of DAE to the AF mixed feed ameliorated the adverse effect of AF on the serum biochemical values by causing increase in serum total proteins, albumin, triglycerides and cholesterol levels. In addition, a significant increase was recorded in AST, ALT, ALP, BUN and creatinine levels in DAE supplemented broilers. The study concluded that diatomaceous earth is an effective adsorbent to decrease the negative effects of aflatoxin in broiler chicken
Salmonella infections are an important cause of mortality and morbidity in subclinically infected bovines. Bovines thus constitute an important reservoir for human infections. Interestingly, despite decades of research into salmonellosis, the disease and its public health consequences are not really resolved.Salmonella is transmitted to animals and humans through the fecal-oral route. Animals
Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation was studied at various nutrient concentrations and incubation time. Highest colonyforming units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of incubation. The BF cells could be completely inactivated at 80°C in 10 min and with 10% formalin in 24 h. SDS-PAGE profile of BF cells revealed repression of four proteins and expression of three new proteins compared to free cells (FC). The preliminary immune response studies showed that BF cells were superior to FC in stimulating the non-specific immune response of Penaeus monodon.
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