Homo-oligomer DNA strands were immobilized onto silicon/silicon dioxide electrodes using 3-aminopropyltriethoxysilane. These modified substrates were used as working electrodes in a three-electrode electrochemical cell. In-phase and out-of-phase impedances were measured in the range -1 to +1 V with respect to an Ag/AgCl reference electrode, with a superimposed 10 mV ac signal at frequencies of 20 and 100 kHz. Ex situ hybridization with complementary oligomer strands, performed at the surface of modified electrodes, is clearly reflected by negative shifts of about 100 mV in the flat-band potential of the semiconductor. Consecutive hybridization-denaturation steps show that the shifts are reproducible and the process is reversible. The in situ hybridization of complementary strands has also been observed with impedance measurements at Si/SiO 2 substrates and with the use of a field effect device. The direct detection of hybridization with a field effect device was performed under constant drain current mode, and the corresponding variations observed for the gate potential during hybridization are in good agreement with the flat band potential shifts observed with the impedance experiments. Measurements made in the presence of noncomplementary strands demonstrate the selectivity of the device.
A novel method is presented for the specific and direct detection of bacteria using bacteriophages as recognition receptors immobilized covalently onto functionalized screen-printed carbon electrode (SPE) microarrays. The SPE networks were functionalized through electrochemical oxidation in acidic media of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) by applying a potential of +2.2 V to the working electrode. Immobilization of T4 bacteriophage onto the SPEs was achieved via EDC by formation of amide bonds between the protein coating of the phage and the electrochemically generated carboxylic groups at the carbon surface. The surface functionalization with EDC, and the binding of phages, was verified by time-of-flight secondary ion mass spectrometry. The immobilized T4 phages were then used to specifically detect E. coli bacteria. The presence of surface-bound bacteria was verified by scanning electron and fluorescence microscopies. Impedance measurements (Nyquist plots) show shifts of the order of 10(4) Omega due to the binding of E. coli bacteria to the T4 phages. No significant change in impedance was observed for control experiments using immobilized T4 phage in the presence of Salmonella. Impedance variations as a function of incubation time show a maximum shift after 20 min, indicating onset of lysis, as also confirmed by fluorescence microscopy. Concentration-response curves yield a detection limit of 10(4) cfu/mL for 50-microL samples.
Electrochemical impedance measurements were used for the detection of single-strand DNA sequences using a peptide nucleic acid (PNA) probe layer immobilized onto Si/SiO2 chips. An epoxysilane layer is first immobilized onto the Si/SiO2 surface. The immobilization procedure consists of an epoxide/amine coupling reaction between the amino group of the PNA linker and the epoxide group of the silane. A 20-nucleotide sequence of PNA was used. Impedance measurements allow for the detection of the changes in charge distribution at the oxide/solution interface following modifications to the oxide surface. Due to these modifications, there are significant shifts in the semiconductor's flat-band potential after immobilization and hybridization. The results obtained using this direct and rapid approach are supported by fluorescence measurements according to classical methods for the detection of nucleic acid sequences.
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