The goal of the present work is a systematic study on an influence of a strain rate on the mechanical response and microstructure evolution of the selected titanium-based materials, i.e., commercial pure titanium, Ti-6Al-4V alloy with lamellar and globular microstructures produced via a conventional cast and wrought technology, as well as Ti-6Al-4V fabricated using blended elemental powder metallurgy (BEPM). The quasi-static and high-strain-rate compression tests using the split Hopkinson pressure bar (SHPB) technique were performed and microstructures of the specimens were characterized before and after compression testing. The strain rate effect was analyzed from the viewpoint of its influence on the stress–strain response, including the strain energy, and a microstructure of the samples after compressive loading. It was found out that the Ti-6Al-4V with a globular microstructure is characterized by high strength and high plasticity (ensuring the highest strain energy) in comparison to alloy with a lamellar microstructure, whereas Ti6-Al-4V obtained with BEPM reveals the highest plastic flow stress with good plasticity at the same time. The microstructure observations reveal that a principal difference in high-strain-rate behavior of the tested materials could be explained by the nature of the boundaries between the structural components through which plastic deformation is transmitted: α/α boundaries prevail in the globular microstructure, while α/β boundaries prevail in the lamellar microstructure. The Ti-6Al-4V alloy obtained with BEPM due to a finer microstructure has a significantly better balance of strength and plasticity as compared with conventional Ti-6Al-4V alloy with a similar type of the lamellar microstructure.

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Kombucha microbial community (KMC) produces a cellulose-based biopolymer of industrial importance and a probiotic beverage. KMC-derived cellulose-based pellicle film is known as a highly adaptive microbial macrocolony-a stratified community of prokaryotes and eukaryotes. In the framework of the multipurpose international astrobiological project "BIOlogy and Mars Experiment (BIOMEX)," which aims to study the vitality of prokaryotic and eukaryotic organisms and the stability of selected biomarkers in low Earth orbit and in a Mars-like environment, a cellulose polymer structural integrity will be assessed as a biomarker and biotechnological nanomaterial. In a preflight assessment program for BIOMEX, the mineralized bacterial cellulose did not exhibit significant changes in the structure under all types of tests. KMC members that inhabit the cellulose-based pellicle exhibited a high survival rate; however, the survival capacity depended on a variety of stressors such as the vacuum of space, a Mars-like atmosphere, UVC radiation, and temperature fluctuations. The critical limiting factor for microbial survival was high-dose UV irradiation. In the tests that simulated a 1-year mission of exposure outside the International Space Station, the core populations of bacteria and yeasts survived and provided protection against UV; however, the microbial density of the populations overall was reduced, which was revealed by implementation of culture-dependent and culture-independent methods. Reduction of microbial richness was also associated with a lower accumulation of chemical elements in the cellulose-based pellicle film, produced by microbiota that survived in the post-test experiments, as compared to untreated cultures that populated the film. Key Words: BIOlogy and Mars Experiment (BIOMEX)-Kombucha multimicrobial community-Biosignature-Biofilm-Bacterial cellulose. Astrobiology 17, 459-469.

Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha microbial community (KMC) samples, in which Komagataeibacter intermedius was a dominant community-member, were exposed under simulated Martian factors (i.e., pressure, atmosphere, and UV-illumination) outside the International Space Station (ISS) for 1.5 years. In this study, we have determined that OMVs from post-flight K. intermedius displayed changes in membrane composition, depending on the location of the samples and some other factors. Membrane lipids such as sterols, fatty acids (FAs), and phospholipids (PLs) were modulated under the Mars-like stressors, and saturated FAs, as well as both short-chain saturated and trans FAs, appeared in the membranes of OMVs shed by both post-UV-illuminated and "dark" bacteria. The relative content of zwitterionic and anionic PLs changed, producing a change in surface properties of outer membranes, thereby resulting in a loss of interaction capability with polynucleotides. The changed composition of membranes promoted a bigger OMV size, which correlated with changes of OMV fitness. Biochemical characterization of the membrane-associated enzymes revealed an increase in their activity (DNAse, dehydrogenase) compared to wild type. Other functional membraneassociated capabilities of OMVs (e.g., proton accumulation, interaction with linear DNA, or synaptosomes) were also altered after exposure to the spaceflight stressors. Despite alterations in membranes, vesicles did not acquire endotoxicity, cytotoxicity, and neurotoxicity.

This work describes fabrication and characterization of two types of surfaceenhanced Raman scattering (SERS) substrates based on starlike gold nanoparticles (NPs). Type I SERS substrates are formed by adsorption of gold nanoparticles from chemically synthesized colloidal solution onto the modified glass substrates. Type II SERS substrates are fabricated by the direct nucleation and growth of Au NPs on the modified glass substrates immersed in the precursors solution. Morphology, optical, and SERS characteristics of both types of substrates are studied. Study shows that by varying the diameters of nanostars cores and the lengths of their spikes during the synthesis one can tune the plasmon absorption peak position from visible to near-infrared and achieve the resonance with most of common laser wavelengths. The measured SERS enhancement factors are equal to 10 5 and 10 6 for the substrates of type I and type II, respectively. The modeling of the electric field distribution in the vicinity of nanoparticles are done for exciting laser wavelengths varied in the range from 400 to 1000 nm. Calculated electric field distribution in the vicinity of Au NPs at different λ exc correlates with the SERS enhancement factors obtained experimentally.