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T he enrichment or spiking of sewage sludge withThe enrichment or spiking of sewage sludge with heavy metals for heavy metals for experimental purposes is a fairly experimental purposes is a fairly widely used, although sometimes widely used technique (for example, Davis and Carltoncontroversial, technique. A study was undertaken, using a sequential Smith, 1981; Street et al., 1977; Coppola et al., 1988; fractionation scheme, to assess the degree of incorporation of Cu, Ni, Forge et al., 1993; Obbard et al., 1993). Spiking may be and Zn into sewage sludge samples spiked with these metals and undertaken to (i) increase the rate of buildup of metals as those metals already present in the sludge. This is important because there is evidence that the bioavailability of metals added to soil as metal salts may differ

The main objective of this study was to compare the effectiveness of different methods (heavy metals in pore water (PW), diffusive gradients in thin films (DGT), diethylene triamine pentaacetic acid (DTPA) extraction, and total heavy metals (THM) in soil) for the assessment of heavy metal bioavailability from soils having various properties and heavy metal contents. The effect of soil heavy metal pollution on shoot yield and sulfatase enzyme activity was also studied. Wheat (Triticum aestivum) was grown in different soils from Spain (n=10) and New Zealand (n=20) in a constant environment room for 25 days.