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The mechanism of interaction between land use change and landscape pattern is pivotal in research concerning global environmental change. Quantitative evaluation of land use change that has resulted from excessive land development and its impact on landscape pattern are important for sustainable utilization of local land resources. The study area was the Manas River watershed, a typical watershed in an arid area. The present study evaluated the influence of long-term land use change on the landscape pattern; the findings are valuable for economic development and environmental protection. The processes for land use change and changes in landscape pattern were reconstructed and analyzed for data in topographic maps dated 1962 and in remote sensing images taken in 1976, 1989, 1999 and 2008 using mathematical models and landscape metrics. The results indicate significant changes in land use over the past 50 years, with increases in the areas of cultivated land and construction land, and a significant decrease in unused land and woodland areas; grassland and water resources first increased and then tended to decrease over this time. The comprehensive index of land use change is 0.55, indicating an unbalanced, one-way transition in the study area. Landscape fragmentation and diversity increased and landscape dominance decreased; in other words, differences between landscapes decreased.The changes resulted in a significant tendency toward diversity and fragmentation of the landscape. Therefore, large-scale intensive agricultural development can reduce the negative influence of land use change on the landscape pattern.