Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based onRAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistantbacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this researchwas to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNAPolymeraseChain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result ofmedia selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung,Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extractedand then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominantclusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolatesBPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, andEnterobacter bugandensis.
Paddy var Rojoleleis asuperior paddy come from Klaten that released by Department of Agriculture in 2003. Its superior properties are resistant to pests leaf hoppers, fluffier, and fragrant. To increase the productivity of paddy that are of ten used by farmers is to use chemical-based fertilizers. The use of these chemicals will effect to adisruption of ecosystem balancing, reduction the amount of soil microflora which essential forplants. Endophytic bacteria are symbiotic microorganisms living within plant tissues, and does not cause negative effects on the host plant. Endophytic bacteria have a capability increasing crop productivity by producing growth hormone, contributes to plant health, and as bio-control agents. Some endophytic bacteria which contribute to plant growth are: Pseudomonas sp., Enterobacter sp., Staphylococcus sp., Azotobacter sp., And Azospirilum sp., Whereas endophytic bacteria that contribute to the health and plant protection several of them are: Pseudomonas sp., Serratia sp. ,Clavibacter sp., and Bacillus sp. This study was conducted to investigate potential of endophytic bacteria to increase Paddy var Rojolele productivity based on its ability to produce extracellular enzymes and resistance to multiple types of antibiotics. The method were endophytic bacteria isolation from three Paddy varRojolele plants, extracellular enzymes detection and antibiotic resistance testing to chloramfinekol, ampicillin and kanamycin. As the result, 43isolateswere isolated from Paddy var Rojolele. Four isolatesamong them havethe ability to produce extra cellular enzym esandresistant toampicillin, kanamycin, and chloramfinekol. Extra cellular enzyme production capability and resistance to antibiotics makes endophytic bacteria are potentialto improveplant health and also asbio-control agentwhich then willaffect to the productivity of rice. To further ensure its potential to plant, more research is needed.
Abstract. Ahaddin AY, Budiarti S, Mustopa AZ, Darusman HS, Triratna L. 2021. Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity. Biodiversitas 22: 227-232. Lactobacillus plantarum S34 belongs to the Lactobacilli class produces a bacteriocin called plantaricin. Bacteriocins are well known as active compounds that inhibit bacterial growth. This study was conducted to determine the antimicrobial activity of plantaricin S34 and its safety profile in the ddY mouse animal models. Plantaricin 34 from the crude extract was identified using Tricine SDS-PAGE. Antimicrobial activity was observed using disk diffusion against EPEC K1.1, S. aureus, S. typhosa, S typhimurium, and Proteus sp. The safety assessment showed that crude extract of plantaricin S34 did not cause any abnormalities to experimental mice even after being administrated with 5000 mg/kg BB. The identification of plantaricin S34 showed an active molecule at 7.34 kDa and had an activity to inhibit the pathogens used in this study. The blood analysis showed that the hematological and biochemical blood properties were in the normal range. Histopathological examination showed no damage to the intestine, liver, and kidneys. Thus, the crude extract of plantaricin S34 is active as an antimicrobial agent without any toxicity effects.
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