We studied parental provisioning and chick growth rates of Cassin's auklet Ptychoramphus aleuticus at Triangle Island, British Columbia, Canada, from 1996 to 1999. Auklet reproductive performance and ocean climate conditions during these years were highly variable, and reflected in the diet composition. Chick growth was maximal when the diet was predominated by copepods, in particular Neocalanus cristatus. Provisioning and growth were high in 1999, intermediate in 1997 and poor in 1998. Exceptional was 1996, when growth was low but provisioning rates were high. Provisioning and growth were depressed late in 1997 and throughout 1998 when larval rockfish Sebastes spp. (5200 cal g ) in the nestling diet. Zooplankton surveys indicated that N. cristatus was substantially more abundant in May 1999 than in May 1998, and during 1999 the auklets foraged in areas with the highest concentrations of copepods. Through impacts on prey availability, variation in ocean climate affects the reproductive performance of Cassin's auklet. Performance tends to be favorable in years when spring is late and cold, and poor when spring is early and warm. Equations for predicting food delivery rates from 24 h mass changes of chicks were highly year-specific, precluding their application in other years or at other sites where Cassin's auklets breed. Between-year differences were also found in relationships between adult provisioning and chick growth. These were strongly positively related in 1999, positively related in 1996 and 1997, but unrelated in 1998; differences attributed to the magnitude of temporal variation in the nestling diet. Finally, we detected annual differences in parental response to chick needs. In 1999, parents delivered more food to chicks in poor condition and less to those in better condition, responses not observed in 1998. Different responses between years may have reflected variation in the availability of prey.

Comparative global proteome analyses were performed on Leptospira interrogans serovar Copenhageni grown under conventional in vitro conditions and those mimicking in vivo conditions (iron limitation and serum presence). Proteomic analyses were conducted using iTRAQ and LC-ESI-tandem mass spectrometry complemented with two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. A total of 563 proteins were identified in this study. Altered expression of 65 proteins, including upregulation of the L. interrogans virulence factor Loa22 and 5 novel proteins with homology to virulence factors found in other pathogens, was observed between the comparative conditions. Immunoblot analyses confirmed upregulation of 5 of the known or putative virulence factors in L. interrogans exposed to the in vivo-like environmental conditions. Further, ELISA analyses using serum from patients with leptospirosis and immunofluorescence studies performed on liver sections derived from L. interrogans-infected hamsters verified expression of all but one of the identified proteins during infection. These studies, which represent the first documented comparative global proteome analysis of Leptospira, demonstrated proteome alterations under conditions that mimic in vivo infection and allowed for the identification of novel putative L. interrogans virulence factors.