A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener.
The sustainable waste management and energy generation from the lignocellulosic biomass is the predominant methodology for meeting the energy requirement globally. Lignocellulosic biomass of Prosopis juliflora (PJ) can be utilized to produce biomethane with the codigestion of activated sludge, a promising alternative energy source. PJ is a perennial, deciduous shrub and an invasive weed in most of the countries and a source of abundant organic material. Utilization of organic waste in the anaerobic digestion process becomes the need of the hour for environmental protection and for energy generation. In this study, NaOH pretreatment of PJ was performed to remove maximal lignin content, which increases the substrate porosity with least sugar loss. The study of the batch anaerobic digestion of the carbon-rich substrate PJ was conducted at a mesophilic temperature of 37 ± 2 °C with six different feedstock (PJ) proportions. The physicochemical properties of the feedstock revealed the presence of nutrients needed by methanogenic bacteria. The maximum biogas yields from 6 different ratios of feedstocks were 15.0
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