The climatic conditions in Croatia are deteriorating which significantly increases the frequency of heat stress. This creates a need for an adequate dairy farming strategy. The impact of heat stress can be reduced in many ways, but the best long-term solution includes the genetic evaluation and selection for heat stress resistance. In order to create the basis for genetic evaluation, this research determined the variation in daily milk yield (DMY) and somatic cell count (SCC) as well as the differences in resistance to heat stress due to production level (high, low) and breed (Holstein, Simmental) of dairy cattle breed in Croatia. For statistical analysis, 1,070,554 test-day records from 70,135 Holsteins reared on 5679 farms and 1,300,683 test-day records from 86,013 Simmentals reared on 8827 farms in Croatia provided by the Croatian Agricultural Agency were used. The results of this research indicate that the high-producing cows are much more susceptible to heat stress than low-producing especially Holsteins. Also, the results of this research indicate that Simmental breed, in terms of daily milk production and somatic cell count, could be more resistant to heat stress than Holstein. The following research should determine whether Simmentals are genetically more appropriate for the challenges that are in store for the future milk production in this region. Furthermore, could an adequate production level be achieved with Simmentals by maintaining the heat resistance?

The aim of this paper was to develop a national singlestep genomic BLUP that integrates multi-national genomic estimated breeding values (EBV) and associated reliabilities without double counting dependent data contributions from the different evaluations. Simultaneous use of all data, including phenotypes, pedigree, and genotypes, is a condition to obtain unbiased EBV. However, this condition is not always fully met, mainly due to unavailability of foreign raw data for imported animals. In dairy cattle genetic evaluations, this issue is traditionally tackled through the multiple across-country evaluation (MACE) of sires, performed by Interbull Centre (Uppsala, Sweden). Multiple across-country evaluation regresses all the available national information onto a joint pedigree to obtain country-specific rankings of all sires without sharing the raw data. In the context of genomic selection, the issue is handled by exchanging sire genotypes and by using MACE information (i.e., MACE EBV and reliabilities), as a valuable source of "phenotypic" data. Although all the available data are considered, these "multi-national" genomic evaluations use multi-step methods assuming independence of various sources of information, which is not met in all situations. We developed a method that handles this by single-step genomic evaluation that jointly (1) uses national phenotypic, genomic, and pedigree data; (2) uses multi-national genomic information; and (3) avoids double counting dependent data contributions from an animal's own records and relatives' records. The method was demonstrated by integrating multi-national genomic EBV and reliabilities of Brown Swiss sires, included in the InterGenomics consortium at Interbull Centre, into the national evaluation in Slovenia. The results showed that the method could (1) increase reliability of a national (genomic) evaluation; (2) provide consistent ranking of all animals: bulls, cows, and young animals; and (3) increase the size of a genomic training population. These features provide more efficient and transparent selection throughout a breeding program.

Optimization of industrial-scale deodorization of high-oleic sunflower oil (HOSO) via response surface methodology is presented in this study. The results of an experimental program conducted on an industrial-scale deodorizer were analyzed statistically. Predictive models were derived for each of the oil quality indicators (QI) in dependence on the studied variable deodorization process parameters. The deodorization behavior of some minor components was analyzed on a pilot-scale deodorizer. For comparison, a similar experimental program was also performed on the laboratory-scale. The results of this study demonstrate that optimization of the deodorization process requires a suitable compromise between often mutually opposing demands dictated by different oil QI. The production of HOSO with top-quality organoleptic and nutritional values (high tocopherol and phytosterol contents and low free and trans fatty acid contents) and high oxidative stability demands deodorization temperatures in the range between 220 and 235 7C and a total sparge steam above 2.0% (wt/wt in oil). The response surface methodology provides the tools needed to identify the optimum deodorization process conditions. However, the laboratoryscale experiments, while showing similar response characteristics of QI in dependence on the process parameters and thus helpful as a guide, are of limited value in the optimization of an industrial-scale operation.

Abstract. Aiming at the determination of the metabolic disorder prevalence as well as the effect of subclinical disorders on daily milk traits of Holsteins, over 1 million (1 962 831) test day records were analysed. The metabolic disorder (ketosis or acidosis) prevalence was indicated by the fat-to-protein (F /P ) ratio, while subclinical disorder was indicated by the F /P ratio and cows' daily production. The effect of subclinical disorders on daily milk traits was tested by Scheffe's method (SAS/STAT). The highest ketosis prevalence occurred in early lactation. In first-parity cows ketosis prevalence was highest in the first 15 days of lactation, while in cows in higher lactations, the prevalence peak occurred on the 25th day. A higher ketosis prevalence during the entire lactation was observed in multiparous cows compared to first-parity cows. Regarding the acidosis prevalence, high values were determined at the beginning of the lactation with a decreasing trend until mid-lactation, when the prevalence increased up to 22 %. The highest decrease in daily milk yield as a consequence of subclinical ketosis was determined as 4.21 and 3.72 kg day −1 in first-parity cows and those with more than four lactations, respectively. A significant negative effect of subclinical acidosis on daily milk yield (2.79 kg day −1 ) was highest in cows in the third lactation. A production decline in subsequent milk controls due to subclinical ketosis or acidosis in all cows was also determined. Subclinical disorders also significantly alter daily milk quality. This indicates that the test day records could be used as a cost-effective and non-invasive method for monitoring herd health.

This study investigated some carcass and meat quality traits of Boer kids (17 male and 17 female) at two different average slaughter ages (83 and 139 days). Jointed cuts of half carcasses arranged from the greatest to the smallest were: hind leg (28.5%), rib and flank (21.2%), shoulder (19.3%), back (8.5%), loin (7.9%), neck (7.6%) and chuck (3.4%). Male kids had significantly higher percentage of the neck cuts (p≤0.001) while females had significantly higher percentage of rib and flank cuts (p≤0.05). At higher slaughter ages neck (p≤0.05) and chuck (p≤0.001) percentages significantly decreased and rib and flank (p≤0.001) percentage significantly increased. On average, hind leg had 72.2 % of muscle, 8.6 % of fat and 18.8 % of bone. Female kids had higher muscle and lower bone hind leg content than males (p≤0.01). Hind leg bone content significantly decreased at higher slaughter age (p≤0.01). Meat from male kids displayed significantly higher cie L*(p≤0.001) and b*(p≤0.05) values than females. At higher slaughter age L* values significantly decreased (p≤0.01) while a* and b* values significantly increased (p≤0.001; p≤0.01)

No abstract

Analysis of longevity in Slovenian holstein cattle The longevity of Slovenian Holstein population was analysed using survival analysis with a Weibull proportional hazard model. Data spanned the period between January 1991 and January 2010 for 116,200 cows from 3,891 herds. Longevity was described as the length of productive life-from first calving till culling or censoring. Records above the sixth lactation were censored to partially avoid preferential treatment. Statistical model included the effect of age at first calving, stage of lactation within parity, yearly herd size deviation, season defined as year, herd, and sire-maternal grandsire (mgs). Some effects had time varying covariates, which lead to 1,839,307 or on average 16 elementary records per cow. Herd and sire-maternal grandsire effects were modelled hierarchically. Pedigree for sires and maternal grandsires included 2,284 entries. Estimated variance between herds was 0.12, while between sire variance was 0.04. Heritability was evaluated at 0.14. Genetic trend for sires was unfavourable, but not significant. A further research is needed to define the required number of daughters per sire and the dynamics of genetic evaluation for sires whose majority of daughters still have censored records.

Considering the rapid climate changes worldwide, with the purpose to reduce financial losses for dairy farmers and enable a more sustainable farming as well, there is an increasing necessity to implement breeding values for heat resistance in breeding strategies. The estimation of breeding values requires a determination of temperature-humidity index (THI) threshold value. Therefore, the objective of this study was to determine the temperature-humidity index (THI) threshold values for daily milk yield, daily fat and protein content of milk produced by Holsteins and dairy Simmentals in Croatian farms. With that purpose, 1.070,554 test-day records from 70.135 Holsteins reared on 5.679 farms and 1.300,683 test-day records from 86.013 Simmentals reared on 8.827 farms in Croatia were compared to records of ambient temperature and relative humidity collected in the barn, during regular milking recordings from January 2005 to December 2012. Accordingly the following conclusions could be drawn: threshold values for daily milk yield were highly dependant on the parity and breed (Holsteins: 68, 69 and 72 for 1 st , 2 nd , 3+ parity; Simmentals: 77 for 1 st ); daily fat and protein contents showed a highly significant decrease due to heat stress condition (THI in 66-80) in both, Holstein and Simmental cows, and in all parity classes; multiparous cows were more resistant to heat stress than primiparous; Simmental cows were more resistant to heat stress than Holsteins.