A new double immunohistochemical technique for the simultaneous detection of B-cells and T-cells was investigated, using tissue preparations obtained from human axillary lymph nodes and rejected renal allografts. The specimens were immunostained first for the demonstration of B-cells, by the immunogold-silver staining (IGSS) method using Leu-12 monoclonal antibody, and then for T-cells by the avidin-biotin-peroxidase complex (ABC) method using Leu-1 monoclonal antibody. With the present methods, both B-cells and T-cells were clearly detected and distinctively identified without cross-linking of antibodies or double reaction of enzymes.
Sepsis is a critical syndrome and DIC often develops in severe septicemia. However, cares for severe patients are limited in remote hospitals. In addition, bad weather often makes medical evacuation difficult in such areas. A 66‐year‐old man had urinary tract infection by
Escherichia coli
, followed by septic shock and DIC rapidly just 2 days after the onset. He recovered in 3 weeks without any massive bleeding. Immediate insertion of the central venous catheter to maintain stable hemodynamics and Gram‐staining for selecting antibiotics were considered essential techniques for the survival of sepsis patients even in remote hospitals.
A variety of tumors arising in the neck often provides a difficulty in the diagnosis. Here we present a retrospective clinicopathological study of 108 tumors on the neck except those due to thyroid or parathyroid diseases, in which histological diagnosis could be achieved by biopsy or surgery during a past 12-year period. An analysis of the causes revealed that tumors were as predominant as 73.1% (79 cases) followed by inflammation (15.7%) and congenital diseases (9.3%); and malignant diseases accounted for 15.7%. As to the locations, median cervical cyst was predominant on the anterior region of neck; tumor of salivary gland, on the bilateral regions of neck; and atheroma, lymphoma, and calcified epitherioma, on the posterior region of neck. There were 25 tumors of salivary gland including 20 tumors of parotid gland, 12 pleomorphic adenomas, 3 Warthin tumors, one lypoma, and 2 squamous cell carcinomas. Five tumors of submandibular gland were all pleomorphic adenomas. Neurinomas arised in the hypoglossal nerve in 2 and phrenic nerve in one cases. Thirty-three cases of lymphonodus diseases were comprised of 39.4% of simple lymphadenitis, 24.2% of metastasized cancer, 18.2% of malignant lymphoma and the like, 9.1% of nodular lymphadenitis, and the others. Congenital diseases included median cervical cyst in 6 cases, cyst on lateral region of neck in 3, cystic lymphangioma in one.-8-
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