The investigations of the platelet-derived biologic agents in osteoarthritis treatment had shown promising but often controversial Abstracts / Osteoarthritis and Cartilage 25 (2017) S76eS444 S438
Purpose: Cartilage is a tough, flexible tissue found throughout the body. It can become damaged because of a sudden injury, such as a sports injury, or gradual wear and tear (osteoarthritis). Injured articular cartilage has a poor regenerative capability, for this reason, tissue engineering may provide a fundamental therapeutic solution to repair cartilage injury. Human articular cartilages are composed of dense extracellular matrix and chondrocytes. One of the major components in the extracellular matrix is hyaluronic acid. The combination of hyaluronic acid with other natural polymers to prepare hydrogel scaffolds may be potentially useful in many tissue engineering applications, including cartilage regeneration. We propose the synthesis of hydrogels based on hyaluronic acid and chitosan by a new strategy of synthesis using diisocyanates to obtain an interpenetrated network of chitosan and hyaluronic acid. Methods: Different kinds of hydrogels were synthesized using hyaluronic acid (Bioib erica, Spain), natural polymers (gelatine, chitosan) and stabilized with diisocianates. The hydrogels were charged with chondroitin sulfate (Bioib erica, Spain). Morphological Characterization: The hydrogels were characterized by scanning electron microscopy and energy dispersive X-ray microscopy in order to study the morphology and the composition of the hydrogels. Hydrogel Swelling: Dynamic swelling experiments were performed by placing discs of stabilized hydrogels in PBS at 37.0 C, and measuring their weight gain as a function of time. Biocompatibility: Cytotoxicity in vitro assays (MTT) and cell adhesion tests (Alamar Blue) were performed on the hydrogels using different cell lines to corroborate the biocompatibility of the hydrogels. Results: All the synthesized hydrogels present a high interconnected porosity. The studied hydrogels swell around 200%. Aaround five hours equilibrium is reached and this equilibrium depend on the ratio hyaluronic acid/natural polymer increasing with the amount of hyaluronic acid present on the hydrogel. MTT assay indicated that the extracts collected at different times from the hydrogels are not cytotoxic and could be considered biocompatible. Cell adhesion assay indicates that the hydrogels support cell adhesion and proliferation, and this behaviour is better on SC-loaded hydrogels. Conclusions: Hydrogels based on hyaluronic acid and natural polymers are proposed as scaffolds for tissue engineering. The presence of interconnected porous and the good swelling properties and biocompatibility represent a great advantage for application in cartilage regeneration. The addition of drugs or glycosaminoglycan is being studied to improve the cartilage regeneration.
3'-SIALLYLACTOSE PROMOTES CARTILAGE REGENERATION BY EXTRACELLULAR MATRIX REMODELING
For the first time the results of a comparative evaluation of low-molecular weight hyaluronic acid (synocrom forte) and autologous conditioned serum (ACS) in coxarthrosis (CA) are presented. Throughout the 12-month monitoring we examined 54 patients with CA who were divided into 2 groups of comparable age and sex composition and the duration of the disease. Group №1 had a 3-week course of local therapy of synocrom forte and group №2 - of ACS. It is concluded that the use of ACS at CA to achieve favorable clinical effects is almost as good as hyaluronic acid, but it characterized by a greater duration of their conservation (from six to twelve months).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.