Chloroplasts are commonly the site of the earliest abiotic injury visible in plant ultrastructure. In this study, six inbred lines of maize (Zea mays L.) were used to analyze changes in the ultrastructure of chloroplasts and related physiological parameters under conditions of drought stress simulated by 20% polyethylene glycol 6000 (-0.6 MPa) for three days. Chloroplasts of three maize lines proved to be more sensitive. They showed changes in ultrastructure in response to drought, including damage to thylakoid membranes, an increase in the number and size of plastoglobuli, swelling of thylakoid membranes both stromal and granal, disorganization of the thylakoid membrane system, an obvious increase in the intrathylakoid space, and a decrease in the length-to-width ratio and area of chloroplasts. In addition, the contents of malondialdehyde increased markedly in the sensitive lines. Contrary to the sensitive lines, stable structures and shapes in chloroplasts were observed in the drought-resistant lines; it could be considered as an advantage contributing to drought tolerance in the plants. In addition, the drought index of leaf fresh mass (LMDI) in drought-sensitive lines was ≤ 0.5, which was also associated with a lower content of leaf chlorophyll. In contrast, drought tolerance coincided with lesser growth reduction, and higher LMDI and leaf chlorophyll content.

The human ether-à-go-go-related gene (hERG) K+ channel plays an important role in cardiac action potentials. The inhibition of the hERG channel may lead to long QT syndrome (LQTS) and even sudden cardiac death. Due to severe hERG-related cardiotoxicity, many drugs have been withdrawn from the market. Therefore, it is necessary to estimate the chemical blockade of hERG in the early stage of drug discovery. In this study, we collected 12,850 compounds with hERG inhibition data from the literature and trained a series of hERG blocking classification models based on the MACCS and Morgan fingerprints. A consensus model named HergSPred was generated based on the individual models using voting principles. The accuracy of HergSPred is higher than previous models using identical training and test sets. Moreover, we analyzed the contribution of each input fingerprint to the prediction output to obtain intuitive chemical insights into the hERG inhibition, which allows visualization of warning substructures that may cause cardiotoxicity in the input compound. The model is available at .

Salicylic acid (SA) and nitric oxide (NO) form a new group of plant growth substances that cooperatively interact to promote plant growth and productivity. Water deficit (WD) stress is a major limiting factor for photosynthesis, which in turn limits crop yield. However, the mechanism of SA and NO in stimulating photosynthesis has not yet been elucidated. Therefore, in this study, we investigated the SA-and NO-mediated photosynthetic adaptability of maize seedlings to WD in terms of photosynthetic parameters, activities and mRNA levels of CO 2 assimilation enzymes. Our results showed that SA alleviated the WD-induced reduction of photosynthetic performance. The activities of Rubisco and Rubisco activase enzymes increased significantly due to SA pretreatment. Moreover, higher transcription rates of Rbc L, ZmRCAα and ZmRCAβ mRNA further confirmed the effects of SA on CO 2 assimilation. WD or SA-induced decreases or increases of CO 2 assimilation ability were further decreased after c-PTIO addition.

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